Journal Title
Title of Journal: Med Chem Res
|
Abbravation: Medicinal Chemistry Research
|
|
|
|
|
|
|
|
Authors: Leyue Du Jianming Guo Dawei Qian Erxin Shang Shu Jiang Pei Liu Shulan Su Fang Zhang Jinao Duan Jun Xu Min Zhao
Publish Date: 2014/08/13
Volume: 24, Issue: 3, Pages: 1289-1297
Abstract
A sensitive and rapid method for simultaneous determination of baicalin baicalein wogonoside wogonin berberine copstisine and palmatine of Scutellaria baicalensis and Coptidis rhizoma extract in rat plasma based on ultra highperformance liquid chromatography coupled with tandem mass spectrometry UPLC–MS/MS was developed Plasma samples were processed by protein precipitation with methanol Chromatographic analysis was performed on an Acquity BEH C18 column 100 mm × 21 mm 17 µm using a mobile phase consisting of acetonitrile and water containing 01 formic acid Electrospray ionization in positive ion mode and multiple reaction monitoring were used to identify and quantify active components All calibration curves gave good linearity r 0991 over the concentration range from 072 to 375 ng/mL for all components Recovery was 5811–10283 depending on the analyte and interday variability was less than 13 The lower limit of quantification for all compounds was between 082 and 133 ng/mL The intra and interday precision and accuracy of the quality control samples at three concentrations showed ≤147 relative standard deviation and 866–1055 accuracy The validated method was successfully applied to determine the concentrations of seven bioactive components in rat plasma after oral administration of Radix scutellariae and Coptidis rhizoma extractThis work was financially supported by National Basic Research Program of China 973 Program 2011CB505300 2011CB505303 the Nature Science Foundation of China No 81072996 81102743 Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions PAPD Construction Project for Jiangsu Key Laboratory for High Technology of TCM Formulae Research BM2010576
Keywords:
.
|
Other Papers In This Journal:
- Synthesis and anti-inflammatory activity evaluation of novel 3-alkyl-6-(4 H -1,2,4-triazol-4-yl)-3,4-dihydro-2 H -benzo[ e ][1,3]oxazine derivatives
- Antimalarial and free radical scavenging activities of rhizomes of Polygonatum verticillatum supported by isolated metabolites
- Design, synthesis, and in vitro antimicrobial activities of novel azetidinyl-3-quinazolin-4-one hybrids
- Novel GABA-AT inhibitors: QSAR and docking based virtual screening of phenyl substituted β-phenyl ethylidene hydrazine analogues
- Synthesis and anticancer activity study of indolyl hydrazide–hydrazones
- Ulcerogenicity devoid novel non-steroidal anti-inflammatory agents (NSAIDS): syntheses, computational studies, and activity of 5-aryliden-2-imino-4-thiazolidinones
- Synthesis, characterization, and SAR studies of new (1 H -indol-3-yl)alkyl-3-(1 H -indol-3-yl)propanamide derivatives as possible antimicrobial and antitubercular agents
- Synthesis, characterization, and SAR studies of new (1 H -indol-3-yl)alkyl-3-(1 H -indol-3-yl)propanamide derivatives as possible antimicrobial and antitubercular agents
- Synthesis, characterization, and anticancer studies of S and N alkyl piperazine-substituted positional isomers of 1,2,4-triazole derivatives
- Expedient synthesis of some novel pregnane derivatives and their evaluation as anti-oxidant and anti-dyslipidemic agents
- Liquid chromatographic analysis of halofantrine from dosage form and its metal interaction studies
- Porphyrin interaction with the membrane: the use of membrane models to elicit the diverse partition in normal and neoplastic tissue
- 2D and 3D QSAR analyses to predict favorable substitution sites in anilino-monoindolylmaleimides acting as PKCβII selective inhibitors
- Synthesis and neuroprotective evaluation of ( E )-3,4-dihydroxystyryl p -substituted-phenethyl ketone derivatives against inflammatory and oxidative injury
- Synthesis of some novel triazoloquinazolines and triazinoquinazolines and their evaluation for anti-inflammatory activity
- Magnetic resonance assays of haloperidol in human serum albumin
- Synthesis of hydrophobic N -acylated isonicotinic acid hydrazide derivatives as potential enoyl–acyl carrier protein reductase (InhA) inhibitors
- Bioactive chemical constituents from the root of Clerodendrum phlomidis
- Synthesis of N -(3-picolyl)-based 1,3,2λ 5 -benzoxazaphosphinamides as potential 11β-HSD1 enzyme inhibitors
- An efficient anticancer histone deacetylase inhibitor and its analogues for human HDAC8
- Mannich base derivatives of 1,3,4-oxadiazole: synthesis and screening against Entamoeba histolytica
- Antimicrobial and cytotoxic evaluation of eugenol derivatives
- Synthesis and cytotoxic evaluation of 7-chloro-4-phenoxyquinolines with formyl, oxime and thiosemicarbazone scaffolds
- Novel metal complexes, their spectrophotometric and QSAR studies
- Novel derivatives of 5-amino-1-cyclopropyl-7-[(3 R ,5 S )3,5-dimethylpiperazine-1-yl]-6,8-difluoro-4-oxo-quinoline-3-carboxylic acid: their synthesis, antimicrobial, antifungal, and urease inhibitory studies
- Synthesis of novel 4,6-diaryl-2-aminopyrimidines as potential antiplasmodial agents
- 2-(2′-Pyridyl) benzimidazole derivatives and their urease inhibitory activity
- Structure-based predictive model for some benzimidazole inhibitors of hepatitis C virus NS5B polymerase
- Synthesis, anti-inflammatory evaluation, and docking studies of some new thiazole derivatives
- A QSAR classification study on inhibitory activities of 2-arylbenzoxazoles against cholesteryl ester transfer protein
- Synthesis of novel 4-hydroxycoumarin derivatives: evaluation of antimicrobial, antioxidant activities and its molecular docking studies
- Synthesis and evaluation of some semicarbazone- and thiosemicarbazone-based cathepsin B inhibitors
- Pharmacophore modeling, virtual screening, and 3D-QSAR studies on a series of non-steroidal aromatase inhibitors
- Synthesis and antimalarial evaluation of novel pyridine quinoline hybrids
- In vitro and in silico exploration of IL-2 inhibition by small drug-like molecules
- Quantum chemical QSAR study of flavones and their radical-scavenging activity
- Synthesis and biological evaluation of novel pyrimidine-2(1 H )-ones/thiones as potent anti-inflammatory and anticancer agents
- Microwave-assisted synthesis of novel 4 H -chromene derivatives bearing 2-aryloxyquinoline and their antimicrobial activity assessment
- Indazole: a medicinally important heterocyclic moiety
- Effect of garlic and cabbage on healing of gastric ulcer in experimental rats
- Structure and toxicity of clozapine and olanzapine on agranulocytosis
- Comparative QSTR study of saturated alcohols based on topological, constitutional, geometrical, and getaway descriptors
- Synthesis and antihypertensive activity evaluation in spontaneously hypertensive rats of nitrendipine analogues
- Green synthesis and biological evaluation of some new benzothiazolo [2,3- b ] quinazolin-1-ones as anticancer agents
- Analysis of the essential oil from the aerial parts of Psoralea pubescence (Miq.) Standl and its antibacterial activity
- Synthesis, toxicity and chemo-sensitization of HeLa cells to etoposide, of some 2-methyl amino acid ester-substituted-1,3-benzoxazines
- Three-dimensional quantitative structure–activity relationship (3D-QSAR) analysis and molecular docking of ATP-competitive triazine analogs of human mTOR inhibitors
- Design and synthesis of new (bis)trifluoromethyl-promoted N -aryl biquinoline derivatives as antitubercular and antimicrobial agents
- Synthesis, characterization, and in vitro anti- Mycobacterium tuberculosis activity of terpene Schiff bases
- Phytochemical characterization and effect of Calendula officinalis , Hypericum perforatum , and Salvia officinalis infusions on obesity-associated cardiovascular risk
- Synthesis and in vitro antimicrobial evaluation of novel 2-amino-6-(phenylthio)-4-(2-(phenylthio)quinolin-3-yl)pyridine-3,5-dicarbonitriles
- Synthesis and cytotoxic evaluation of novel 2,3-di- O -alkyl derivatives of l -ascorbic acid
- Solvent- and catalyst-free synthesis of bis-adducts of 3-formylchromone as potential antimicrobial agents
- Synthesis of novel 2-(3′-aryl-sydnon-4′-ylidene)-5′-substituted-[1,3,4]-thiadiazolylamines and [1,3,4]-thiadiazol-2′-yl-3-oxo-[1,2,4]-triazoles as antimicrobial agents
- Molecular modeling design, synthesis, and anti-hyprglycemic evaluation of certain 5-(aryl-alkoxy-benzylidine)-imidazolidine-2,4-dione derivatives as potential PPARγ agonists
- Design, synthesis and neuraminidase inhibitory activity of N -(5-benzyl-4-( tert -butyl)thiazol-2-yl)benzamides
- Synthesis, characterization and in vitro hydrolysis studies of ester and amide prodrugs of dexibuprofen
- Transformations of naproxen into pyrazolecarboxamides: search for potent anti-inflammatory, analgesic and ulcerogenic agents
- Antituberculosis activity of hydrazones derived from 4-fluorobenzoic acid hydrazide
- Indolizine: a biologically active moiety
- Design, synthesis, and evaluation of the anticonvulsant and antidepressant activities of pyrido[2,3- d ]pyrimidine derivatives
- Synthesis and evaluation of novel benzene-ethanol bearing
1,2,4-triazole derivatives as potential antimicrobial agents
- Synthesis, anti-inflammatory activity, and QSAR study of some Schiff bases derived from 5-mercapto-3-(4′-pyridyl)-4 H -1,2,4-triazol-4-yl-thiosemicarbazide
|