Authors: Tao Tao Chen Chen Jie Sun YaJing Peng MinSheng Zhu
Publish Date: 2015/04/11
Volume: 58, Issue: 4, Pages: 373-378
Abstract
Class III βtubulin Tubb3 is a component of the microtubules in neurons and contributes to microtubule dynamics that are required for axon outgrowth and guidance during neuronal development We here report a novel bacterial artificial chromosome BAC transgenic mouse line that expresses Class III βtubulin fused to mCherry an improved monomeric red fluorescent protein for the visualization of microtubules during neuronal development A BAC containing Tubb3 gene was modified by insertion of mCherry complementary DNA downstream of Tubb3 coding sequence via homologous recombination mCherry fusion protein was expressed in the nervous system and testis of the transgenic animal and the fluorescent signal was observed in the neurons that located in the olfactory bulb cerebral cortex hippocampal formation cerebellum as well as the retina Besides Tubb3mCherry fusion protein mainly distributed in neurites and colocalized with endogenous Class III βtubulin The fusion protein labels Purkinje cell dendrites during cerebellar circuit formation Therefore this transgenic line might be a novel tool for scientific community to study neuronal development both in vitro and in vivoThis article is published under license to BioMed Central Ltd Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use distribution and reproduction in any medium provided the original authors and source are creditedThis article is published under an open access license Please check the Copyright Information section for details of this license and what reuse is permitted If your intended use exceeds what is permitted by the license or if you are unable to locate the licence and reuse information please contact the Rights and Permissions team
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