Authors: Jinying Peng Bo Xia Chengqi Yi
Publish Date: 2016/01/29
Volume: 59, Issue: 3, Pages: 219-226
Abstract
Epigenetic changes caused by DNA methylation and histone modifications play important roles in the regulation of various cellular processes and development Recent discoveries of 5methylcytosine 5mC oxidation derivatives including 5hydroxymethylcytosine 5hmC 5formylcytsine 5fC and 5carboxycytosine 5caC in mammalian genome further expand our understanding of the epigenetic regulation Analysis of DNA modification patterns relies increasingly on sequencingbased profiling methods A number of different approaches have been established to map the DNA epigenomes with singlebase resolution as represented by the bisulfitebased methods such as classical bisulfite sequencing BSseq TABseq TETassisted bisulfite sequencing oxBSseq oxidative bisulfite sequencing and etc These methods have been used to generate baseresolution maps of 5mC and its oxidation derivatives in genomic samples The focus of this review will be to discuss the chemical methodologies that have been developed to detect the cytosine derivatives in the genomic DNAThis article is published under an open access license Please check the Copyright Information section for details of this license and what reuse is permitted If your intended use exceeds what is permitted by the license or if you are unable to locate the licence and reuse information please contact the Rights and Permissions team
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