Authors: Xiaole Qi Xiang Gao Zhen Lu Lizhou Zhang Yongqiang Wang Li Gao Yulong Gao Kai Li Honglei Gao Changjun Liu Hongyu Cui Yanping Zhang Xiaomei Wang
Publish Date: 2016/06/08
Volume: 59, Issue: 7, Pages: 717-723
Abstract
To test whether amino acid mutations in the PBC and PHI loops of VP2 are involved in the replication and virulence of infectious bursal disease virus IBDV a pair of viruses namely the moderately virulent IBDV rGxF9VP2 and the attenuated strain rGt were used Residue mutations A222P PBC and S330R PHI selected by sequence comparison were introduced individually into rGxF9VP2 by using a reverse genetics system In addition the reverse mutation of either P222A or R330S was introduced into rGt The four modified viruses were then rescued and evaluated in vitro CEF cells and in vivo SPF chickens Results showed that A222P elevated the replication efficiency of rGxF9VP2 while P222A reduced that of rGt in CEF cells A mutation at residue 330 did not alter IBDV replication In addition animal experiments showed that a single mutation at either residue 222 or 330 did not significantly influence the virulence of IBDV In conclusion residue 222 in PBC of VP2 is involved in the replication efficiency of IBDV in vitro but does not affect its virulence in vivo further facilitating our understanding of the genefunction of IBDVThis article is published under an open access license Please check the Copyright Information section for details of this license and what reuse is permitted If your intended use exceeds what is permitted by the license or if you are unable to locate the licence and reuse information please contact the Rights and Permissions team
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