Authors: L Chaabane L Tei L Miragoli L Lattuada M von Wronski F Uggeri V Lorusso S Aime
Publish Date: 2015/04/15
Volume: 17, Issue: 6, Pages: 819-828
Abstract
GdF was synthesized following FmocSPPS strategy Binding was measured using soluble fibrin DDE fragment and a dried fibrin assay Contrast efficiency was tested on human and mouse clots and in vivo on Neuro2A tumor model An antithrombotic drug was used to evaluate GdF sensitivity for changes in fibrin availability at the tumor siteThe high relaxivity of GdF 42 mM−1 s−1 per molecule yielded a strong signal enhancement in human and murine clots High contrast was also measured in vivo in Neuro2A tumors with a persistent enhancement in tumor rim and stroma Upon treatment with an antithrombotic drug the contrast uptake was significantly reduced in the tumor area confirming the specificity of the probeThis research was supported by funding from AIRC Investigator Grant—IG 2013 prog N14565 SA ESF COST Action TD1004 is also acknowledged The authors thank Simona Ramponi and Adriana Grotti CRB Bracco Imaging SpA for technical assistance and support with tumor model
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