Authors: Hoon Hyun Maged Henary Tielong Gao Lakshminarayana Narayana Eric A Owens Jeong Heon Lee GwangLi Park Hideyuki Wada Yoshitomo Ashitate John V Frangioni Hak Soo Choi
Publish Date: 2015/06/18
Volume: 18, Issue: 1, Pages: 52-61
Abstract
Three complementary synthetic schemes were used to produce novel zwitterionic chemical structures Physicochemical optical biodistribution and clearance properties were compared to Cy55 a conventional pentamethine indocyanine now used for biomedical imagingZW7001a ZW7001b and ZW7001c were synthesized purified and analyzed extensively in vitro and in vivo All molecules had extinction coefficients ≥199000 M−1 cm−1 emission ≥660 nm and stability ≥99 after 24 h in warm serum In mice rats and pigs ≥80 of the injected dose was completely eliminated from the body via renal clearance within 4 h Either alone or conjugated to a tumor targeting ligand ZW7001a permitted dualchannel high SBR and simultaneous imaging with 800nm NIR fluorophores using the FLARE® imaging systemThis study was supported by the following grants from the National Institutes of Health NCI BRP grant R01CA115296 NIBIB grants R01EB010022 and R01EBcp as well as a grant from the Dana Foundation Program in Brain and ImmunoImaging This paper’s contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH MH would like to thank the Brain and Behavior seed grant from the Neuroscience Institute at Georgia State University and funding was also supplied by the EmoryGeorgia Tech Healthcare Innovation Program We thank David J Burrington Jr for editing and Eugenia Trabucchi for administrative assistance
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