Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Pediatr Surg Int

Search In Journal Title:

Abbravation: Pediatric Surgery International

Search In Journal Abbravation:

Publisher

Springer Berlin Heidelberg

Search In Publisher:

DOI

10.1007/s00012-002-8337-2

Search In DOI:

ISSN

1437-9813

Search In ISSN:
Search In Title Of Papers:

Surfactant proteinD attenuates the lipopolysaccha

Authors: Ryuta Saka Tetsu Wakimoto Fumiko Nishiumi Takashi Sasaki Satoko Nose Masahiro Fukuzawa Takaharu Oue Itaru Yanagihara Hiroomi Okuyama
Publish Date: 2015/10/28
Volume: 32, Issue: 1, Pages: 59-63
PDF Link

Abstract

Necrotizing enterocolitis NEC is a devastating inflammatory disease of preterm infants that may depend on overexpression of tolllike receptor4 TLR4 in the immature intestine Surfactant protein SPD is a member of the collectin family and plays an important role in innate immunity particularly in the airways Although SPD also exists in the intestines little is known about its function This study investigated whether SPD can attenuate the inflammatory response of TLR4overexpressing embryonal intestinal cellsAll experimental procedures were performed using the human intestinal cell line INT407 originally derived from human embryonal intestines Plateletactivating factor PAF reported to be elevated in NEC patients was used to induce TLR4 overexpression in the human embryonal intestinal cell line INT407 TLR4 expression was measured using quantitative realtime PCR Inflammatory responses to PAF 5 µM the TLR4 agonist lipopolysaccharide LPS 100 ng/ml PAF + LPS and PAF + LPS following SPD pretreatment 20 µg/ml were assessed by enzymelinked immunosorbent assay ELISA of interleukin8 IL8 release in pg/mlExpression of TLR4 mRNA mean ± SD was upregulated by PAF 369  ± 28  p  0001 Stimulation with PAF + LPS resulted in higher IL8 release 19593 ± 523 than control 1412 ± 124 LPS 1673 ± 658 or PAF 15272 ± 1294 treatment p  005 Release in response to PAF + LPS 15901 ± 3193 was attenuated by SPD pretreatment 11616 ± 1316 p  005

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. Low mortality in necrotizing enterocolitis associated with coagulase-negative Staphylococcus infection
  2. Low mortality in necrotizing enterocolitis associated with coagulase-negative Staphylococcus infection
  3. Chronic bilious vomiting in children in developing countries due to high bowel obstruction: not always malrotation or tuberculosis
  4. Implantable versus cuffed external central venous catheters for the management of children and adolescents with acute lymphoblastic leukaemia
  5. Effect of insulin-like-growth factor and its receptors regarding lung development in fetal mice
  6. Effect of testicular ischemia-reperfusion on recruitment of neutrophils, E-selectin expression and germ cell apoptosis in the contralateral testis in a rat
  7. Effect of testicular ischemia-reperfusion on recruitment of neutrophils, E-selectin expression and germ cell apoptosis in the contralateral testis in a rat
  8. Expression of vasoactive mediators during mechanical ventilation in nitrofen-induced diaphragmatic hernia in rats
  9. Improvement in the quality of life using both Bianchi’s procedure and the closure of a jejunostomy in a case with short bowel syndrome
  10. Combined free autologous auricular cartilage and fascia lata graft repair for a recurrent tracheoesophageal fistula
  11. Influence of hypoxia-dependent factors on the progression of neuroblastoma
  12. The impact of iatrogenic gastroschisis on pulmonary maturation in the fetal rabbit models of congenital diaphragmatic hernia
  13. Intrathoracic and intraabdominal locations of a cystic benign tumor: congenital etiology due to embryological diaphragm development?
  14. Do not rush into operating and just observe actively if you are not sure about the diagnosis of appendicitis
  15. “Pouch colon patch graft”—an alternative treatment for congenital short colon
  16. Pure esophageal atresia with no gap and associated intra-abdominal calcification
  17. An in vitro study of silicone migration from intravenous fluid tubing
  18. Usage of 64-detector-row spiral computed tomography volumetry in preoperative volume prediction in living donor liver transplantation in children
  19. Is adrenomedullin involved in the pathophysiology of persistent pulmonary hypertension of the newborn?
  20. Novel use of porcine urinary bladder matrix for pediatric pilonidal wound care: preliminary experience
  21. An unexpected diagnosis in children with male phenotype and bilateral nonpalpable gonad: congenital adrenal hyperplasia with female genotype
  22. Intestinal perforation secondary to ingested foreign bodies: a single-center experience with 38 cases
  23. Role of penile biometric characteristics on surgical outcome of hypospadias repair
  24. Spontaneous perforation of the biliary tract and portal vein thrombosis in infancy
  25. Small-bowel continuity: A crucial factor in determining survival in gastroschisis
  26. Laparoscopic resection of intra-abdominal extralobar pulmonary sequestration
  27. Prehospital predictors of risk for pelvic fractures in pediatric trauma patients
  28. Visceral coverage with absorbable mesh followed by split-thickness skin graft in the treatment of ruptured giant omphalocele
  29. Severe hyperphosphatemia after administration of sodium-phosphate containing laxatives in children: case series and systematic review of literature
  30. Neonatal pneumoperitoneum: a critical appraisal of its causes and subsequent management from a developing country
  31. Congenital diaphragmatic hernia in CHARGE syndrome
  32. Idiopathic biliary perforation in a 10-year-old boy
  33. Duplicated proctodeum
  34. To drain or not to drain: an analysis of abscess drains in the treatment of appendicitis with abscess
  35. Critical presentation of pleuropulmonary blastoma
  36. Congenital pyloric atresia and associated anomalies
  37. The area and attachment abnormalities of the gubernaculum in patients with undescended testes in comparison with those with retractile testes
  38. Genetics and developmental biology of oesophageal atresia and tracheo-oesophageal fistula: lessons from mice relevant for paediatric surgeons
  39. Letter to the Editor regarding the article “Anorectal malformations and neurospinal dysraphism: is this association a major risk for continence?”
  40. Perforated appendicitis presenting as appendicoumbilical fistula
  41. A complicated case of bilateral cystic nephroma in a 16-month-old boy
  42. Translation and cultural adaptation of the Hirschsprung’s Disease/Anorectal Malformation Quality of life Questionnaire (HAQL) into Swedish
  43. Efficacy of the circulatory management of an antenatally diagnosed congenital diaphragmatic hernia: outcomes of the proposed strategy
  44. Thoracoscopic repair of congenital diaphragmatic hernia: two centres’ experience with 60 patients
  45. Successful complete resection of Ewing sarcoma arising from the bladder in a 10-year-old boy after chemotherapy
  46. Indications for thoracoscopic repair of congenital diaphragmatic hernia in neonates
  47. Neuroblastoma detected by mass screening: the Tumor Board’s role in its treatment
  48. Three-dimensional liver model based on preoperative CT images as a tool to assist in surgical planning for hepatoblastoma in a child
  49. Three-dimensional liver model based on preoperative CT images as a tool to assist in surgical planning for hepatoblastoma in a child
  50. Duodenal duplication with bile duct obstruction and keratinaceous casts
  51. Beneficial effects of Etanercept on experimental necrotizing enterocolitis
  52. Blockade of the renin–angiotensin system prevents acute and immunologically relevant colitis in murine models
  53. Bowel function after surgery for anorectal malformations in patients with tethered spinal cord
  54. PAF antagonist BN-52021 reduces intercellular adhesion molecule-1 expression and oxidative stress in rats with reperfusion damage due to unilateral testicular torsion
  55. Practice variability exists in the management of high-grade pediatric pancreatic trauma
  56. Prenatal diagnosis of cloacal malformations
  57. Silencing of the MYCN gene by siRNA delivered by folate receptor-targeted liposomes in LA-N-5 cells
  58. HoxB2, HoxB4 and Alx4 genes are downregulated in the cadmium-induced omphalocele in the chick model
Search Result: