Authors: Tomoko Hasegawa Hanako O Ikeda Noriko Nakano Yuki Muraoka Tatsuaki Tsuruyama Keiko OkamotoFuruta Haruyasu Kohda Nagahisa Yoshimura
Publish Date: 2016/01/05
Volume: 60, Issue: 2, Pages: 111-125
Abstract
Photoreceptor layer thickness and the ability to visualize photoreceptor ellipsoid zones were analyzed using SDOCT images and these images were compared with hematoxylin and eosinstained sections and electron microscopy images The a and bwave amplitudes of the ERGs were analyzedIn rd10 mice the photoreceptor layer thickness rapidly decreased and the photoreceptor ellipsoid zone was visible on SDOCT images in 89 and 43 of eyes of 21 and 33dayold mice respectively In rd12 mice the photoreceptor layer gradually thinned and the ellipsoid zone remained visible in 92 of eyes at 19 months Electron microscopy revealed that photoreceptor degeneration had occurred on the inner side of the outer nuclear layer in 21dayold rd10 and 7monthold rd12 mice possibly due to autophagy mechanisms Scotopic ERGs of rd10 mice showed a diminished response at 21 days at 33 days no response was detectable In rd12 mice scotopic ERGs were undetectable at 28 days stimulus intensity 30 cds/m2 Photopic ERGs were nearly undetectable in 28dayold rd10 mice but a small bwave was still recordable in 13monthold rd12 miceOur results demonstrate that visual function deteriorated with photoreceptor degeneration within 1 month in rd10 mice In rd12 mice however the process of visual function deterioration and photoreceptor degeneration was still in progress at 13 months of ageWe thank Hitomi Suetsugu for technical assistance and the Center for Anatomical Pathological and Forensic Medical Research for preparation of the histological sections This study was supported by grants from the Ministry of Education Culture Sports Science and Technology of Japan GrandinAid for Young Scientists No 24791850 and for the Innovative TechnoHub for Integrated Medical BioImaging of the Project for Developing Innovation Systems No 24249082 This study was also supported by grants from the Ministry of Health Labour and Welfare of Japan and Japan Society for the Promotion of ScienceT Hasegawa none HO Ikeda Grant the Astellas Foundation for Research on Metabolic Disorders the Japan Foundation for Applied Enzymology the Uehara Memorial Foundation YOKOYAMA Foundation for Clinical Pharmacology Mochida Memorial Foundation Japan Intractable Diseases Research Foundation Japan Research Foundation for Clinical Pharmacology N Nakano none Y Muraoka none T Tsuruyama None K O Furuta none H Kohda none N Yoshimura grant Nidek Topcon Canon Novartis Japan Bayer Santen Senju honorary Nidek Canon Novartis Japan Bayer Santen Senju and consultant fee Nidek Canon
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