Journal Title
Title of Journal: Arch Toxicol
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Abbravation: Archives of Toxicology
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Publisher
Springer-Verlag
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Authors: RueiMing Chen GongJhe Wu YiTing Tai WeiZen Sun YiLing Lin WenChi Jean TaLiang Chen
Publish Date: 2003/03/12
Volume: 77, Issue: 7, Pages: 418-423
Abstract
Nitric oxide is an active oxidant that contributes to the physiology and pathophysiology of macrophages Propofol has been widely used in intravenous anesthesia It possess antioxidant and immunomodulating effects This study aimed to evaluate the effects of propofol on nitric oxide production in lipopolysaccharideactivated macrophages Exposure of macrophages to propofol 25 50 and 75 µM to lipopolysaccharide 05 1 15 and 2 ng/ml or to a combination of propofol and lipopolysaccharide did not affect cell viability However propofol at 100 µM led to significant cell death P005 The levels of nitrite an oxidative product of nitric oxide were increased in lipopolysaccharidetreated macrophages in a concentrationdependent manner P001 while propofol could concentrationdependently decrease the lipopolysaccharideenhanced nitrite levels P001 Immunoblotting analysis revealed that lipopolysaccharide increased the protein level of inducible nitric oxide synthase iNOS The cotreatment of propofol and lipopolysaccharide significantly reduced this lipopolysaccharideinduced iNOS protein 357±49×103 versus 92±6×103 arbitrary units P001 Analysis by reverse transcriptasepolymerase chain reaction showed that lipopolysaccharide induced mRNA of iNOS but that the inductive effect was inhibited by propofol 95±7×102 versus 30±4×102 arbitrary units P001 This study has demonstrated that propofol at therapeutic concentrations could suppress nitric oxide biosynthesis by inhibiting iNOS expression in lipopolysaccharideactivated macrophages The mechanism of suppression was at a pretranslational levelThe authors express their gratitude to Ms SheauLan Tzeng and Ms WanJu Lee for their technical support and data collection of the experiment This study is supported by grants TMU90Y05A123 from Taipei Medical University and NSC902314B038045 from the National Science Council Taiwan ROC
Keywords:
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