Authors: Kenichiro Inoue Hirohisa Takano Rie Yanagisawa Takamichi Ichinose Miho Sakurai Toshikazu Yoshikawa
Publish Date: 2006/02/16
Volume: 80, Issue: 9, Pages: 614-619
Abstract
Particulate matter PM can exacerbate allergic airway diseases Health effects of PM with a diameter of less than 100 nm called nano particles have been focused We have recently demonstrated that carbon nano particles 14 56 nm exaggerate allergic airway inflammation in mice In the present study we investigated the effects of repeated pulmonary exposure to carbon nano particles on the expression of a variety of cytokines in the absence or presence of allergen in mice ICR mice were divided into six experimental groups Vehicle two sizes of carbon nano particles ovalbumin OVA and OVA + nano particles were administered intratracheally Nano particles increased the lung protein levels of thymus and activationregulated chemokine TARC macrophage inflammatory protein MIP−1α and granulocytemacrophage colonystimulating factor GMCSF in the absence or presence of allergen The enhancement was more prominent with 14 nm of nano particles than with 56 nm of nano particles in overall trend 14 nm nano particle exposure significantly enhanced the lung expressions of interleukin IL2 and IL10 in the presence of allergen as compared with allergen exposure These results suggest that pulmonary exposure to nano particles can induce the lung expression of TARC MIP1α GMCSF in the absence of allergen and can enhance that of TARC MIP1α GMCSF IL2 and IL10 in the presence of allergen The enhancing effects are more prominent with smaller particles
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