Identification of Stress Sensitive Genes in Hypert

Authors: H Takle G Baeverfjord Ø Andersen

Publish Date: 2005/12/02

Volume: 30, Issue: 3-4, Pages: 275-281

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Abstract

Deformities of skeletal structures the heart and other organs are a recurrent problem in species used in intensive aquaculture Elevated egg incubation temperature appears to be a high risk factor in the development of these malformations but the causal relation has not been established Our aim was to identify candidate genes involved in the development of heat induced deformities in Atlantic salmon Salmo salar Temperature sensitive genes were isolated by RNA Arbitrarily Primed RAPPCR A total of 33 RAPPCR products were successfully sequenced and the expression of eight identified genes was further examined by RTPCR from pooled samples of heat exposed embryos Five of these genes were demonstrated to be temperature sensitive of which four were shown to be upregulated and one was downregulated at elevated water temperatures The atrial natriuretic peptide ANP gene which is a promising candidate for heart deformities showed the highest level of heat induction Three additional RAPPCR products identified as heterogeneous nuclear ribonucleprotein hnRNP A0 acyl CoA binding protein ACBP and mitochondrial mtHSP70 showed upregulated mRNA expression in response to elevated water temperature The single downregulated gene was identified as an Atlantic salmon Salmo salar homolog of the cysteine and tyrosinerich 1 CYYR1 gene This study demonstrated that a temperature elevation of only 4 °C during the early stages of the organogenesis in Atlantic salmon induce altered expression of a number of genes which are candidates for the development of heat induced deformities

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