Journal Title
Title of Journal: Fish Physiol Biochem
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Abbravation: Fish Physiology and Biochemistry
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Publisher
Springer Netherlands
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Authors: Yudong Jia Zhen Meng Huaxin Niu Peng Hu Jilin Lei
Publish Date: 2014/06/26
Volume: 40, Issue: 6, Pages: 1639-1650
Abstract
The luteinizing hormone receptor LHR plays a crucial role in female reproduction In the present study fulllength sequence coding for the LHR was obtained from female turbot Scophthalmus maximus by homology cloning and a strategy based on rapid amplification of cDNA endpolymerase chain reaction The fulllength LHR cDNA was 3184 bp long and contained a 2058bp open reading frame which encoded a protein of 685 amino acids Multiple sequence alignments of the turbot LHR manifested high homologies with the corresponding sequences of available teleosts and representative vertebrates and significant homology with that of Hippoglossus hippoglossus In addition the turbot LHR showed typical characteristics of glycoprotein receptors including a long Nterminal extracellular domain seven transmembrane domains and a short Cterminal intracellular domain LHR mRNA was abundant in the ovary but was deficient in extraovarian tissues Furthermore LHR mRNA gradually developed from previtellogenesis to migratory nucleus stage with the highest values observed in migratory nucleus stage during reproductive cycle However LHR mRNA sharply decreased in atresia stage These results suggested that LHR is a typical G proteincoupled receptor that is involved in the promotion of turbot ovarian development and may be related to the final maturation and ovulation of oocyte These findings contribute to the understanding of the potential roles of LHR in controlling the fish reproductive cycleThis study was supported by China Agriculture Research System CRAS50 National Natural Science Foundation of China 31302205 Natural Science Foundation of Shandong Province ZR2012CQ024 and BS2013SW004 and the China Postdoctoral Science Foundation No 2012M511559 and 2013T60690 We thank Xinfu Liu and Chunren Gao Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences for help in the experiment
Keywords:
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