Authors: Shasha Liu Kecheng Zhu Nan Chen Weimin Wang Huanling Wang
Publish Date: 2013/02/08
Volume: 39, Issue: 5, Pages: 1153-1163
Abstract
The ubiquitously expressed hypoxiainducible factor1α HIF1α acts as a key transcription factor in regulating metabolism development cellular survival proliferation and pathology under hypoxia condition Compared to mammals fish are more vulnerable to hypoxia stress and contamination however the regulation of HIF1α in fish remains obscure In this study zebrafish HIF1α promoter was cloned and found to possess a CpG island located at −97 to +403 but the canonical TATAbox was absent Aligning 240bp HIF1α proximal promoter region of zebrafish with other vertebrates showed more than 82 identity with cyprinid fishes Further luciferase analysis suggested that the minimal core promoter might locate at −134 to +97 and several putative transcription factor binding sites were found in this region by bioinformatic analysis Moreover it was shown that the zebrafish HIF1α mRNA was significantly activated by 10 μg/mL lipopolysaccharide LPS under hypoxia condition and peaked at 8 h after treatment suggesting LPS and hypoxiaregulated zebrafish HIF1α transcriptional activity in a synergistic pattern This synergistic effect was closely related to the living environment of fish indicating that this mechanism would be more conducive to fish survival
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