Journal Title
Title of Journal: Fish Physiol Biochem
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Abbravation: Fish Physiology and Biochemistry
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Publisher
Springer Netherlands
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Authors: YungChi Shen Todd Hsu LiBin Ling WenChian You ChiaWei Liu
Publish Date: 2017/01/10
Volume: 43, Issue: 2, Pages: 663-676
Abstract
Nucleotide excision repair NER removes helixdistorting DNA lesions such as UVinduced pyrimidine dimers and cisplatininduced strand crosslinking Our earlier studies have identified lowmolecularweight proteins homologous to the 150kDa vitellogenin 1 Vg1 as UVdamaged DNAbinding factors expressed in developing zebrafish Danio rerio This present study explored if Vg1like proteins also participated in NER in zebrafish Immunoblot analysis of affinitycaptured 12 h postfertilization hpf zebrafish extract proteins showed a transient binding of a 30kDa Vg1like polypeptide to UVdamaged DNA A transcriptionbased in vitro repair assay revealed a significant upregulation of UVC or cisplatinsuppressed transcriptional activity of a marker cDNA driven by a SP6 RNA polymeraseregulated promotor after incubating the damaged plasmid with the extracts of 12 hpf embryos or 96 hpf larvae The upregulation of UV or cisplatinsuppressed transcription was abolished in the presence of a monoclonal antizebrafish Vg1 antibody The differential sensitivity of UVinduced repair in 12 and 96 hpf zebrafish extracts to exogenous ATP suggested a developmentdependent expression of Vg1like NER factors A T4 endonuclease V digestion assay showed no inhibition of the antiVg1 antibody on the excision of UVinduced cyclobutane pyrimidine dimers Our results identified the participation of Vg1like factors in NER in developing zebrafish and these factors may function at postincison steps of NER
Keywords:
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- Spatial learning-induced egr - 1 expression in telencephalon of gold fish Carassius auratus
- Cloning and characterization of the HSP90 beta gene from Tanichthys albonubes Lin (Cyprinidae): effect of copper and cadmium exposure
- Protein and energy utilization and the requirements for maintenance in juvenile mulloway ( Argyrosomus japonicus )
- Evoked potentials in the Atlantic cod following putatively innocuous and putatively noxious electrical stimulation: a minimally invasive approach
- Nutritional evaluation of phosphorylated pumpkin seed ( Cucurbita moschata ) protein concentrate in silver catfish Rhamdia quelen (Quoy and Gaimard, 1824)
- Antioxidative role of selenium against the toxic effect of heavy metals (Cd +2 , Cr +3 ) on liver of rainbow trout ( Oncorhynchus mykiss Walbaum 1792)
- Molecular cloning, characterization, and expression analysis of luteinizing hormone receptor gene in turbot ( Scophthalmus maximus )
- Identification of Stress Sensitive Genes in Hyperthermic Atlantic Salmon ( Salmo salar ) Embryos by RAP-PCR
- Nitrogenous and phosphorus excretions in juvenile silver catfish ( Rhamdia quelen ) exposed to different water hardness, humic acid, and pH levels
- Fish condensate as effective replacer of fish meal protein in diet for striped snakehead, Channa striata (Bloch)
- The effects of cadmium and copper on embryonic and larval development of ide Leuciscus idus L.
- Induction of phase II enzymes and hsp70 genes by copper sulfate through the electrophile-responsive element ( EpRE ): insights obtained from a transgenic zebrafish model carrying an orthologous EpRE sequence of mammalian origin
- Substrate specificity of brain acetylcholinesterase and its sensitivity to carbamate insecticides in Carassius auratus
- Unmethylated CpG oligodeoxynucleotides activate head kidney leukocytes of Atlantic cod, Gadus morhua
- Peptide mapping of polymorphic myosin heavy chain isoforms in different muscle types of some freshwater teleosts
- Universal antisera for immunocytochemical identification of two different gonadotrophs in acanthopterygian fishes
- The isoelectric point, a key to understanding a variety of biochemical problems: a minireview
- Adaptive alterations on gill Na + , K + -ATPase activity and mitochondrion-rich cells of juvenile Acipenser sinensis acclimated to brackish water
- Polymorphisms and DNA methylation level in the CpG site of the GHR1 gene associated with mRNA expression, growth traits and hormone level of half-smooth tongue sole ( Cynoglossus semilaevis )
- Stress-induced inhibition of recruitment of ovarian follicles for vitellogenic growth and interruption of spawning cycle in the fish Oreochromis mossambicus
- Effects of dietary thiamin on the physiological status of the grouper Epinephelus coioides
- Spectral sensitivity of juvenile chub mackerel ( Scomber japonicus ) in visible and ultraviolet light
- The effects of starvation and re-feeding on growth and swimming performance of juvenile black carp ( Mylopharyngodon piceus )
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