Observation of the middle intestinal tight junctio

Authors: Fan Xu YuanTu Ye ChunFang Cai Ping Wu Lin Song Meng Liu LinJie Yao JiaoJiao Dong YuWei Huang Zhi Gong Jie Qin Liang Song

Publish Date: 2014/07/30

Volume: 40, Issue: 6, Pages: 1783-1792

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Abstract

To confirm the existence of the tight junction TJ in middle intestine and obtain the genetic information of Claudin3 Claudin15a Claudinb and Claudinc of grass carp we observed the physical structure of TJ by transmission electron microscopy and cloned the partial cDNAs of the four Claudins using reverse transcriptase PCR technique The four partial cDNAs consist of 1261 490 776 and 662 bp encoded 131 150 195 and 171 amino acids respectively Homology analysis showed that the grass carp Claudin shared high homology with other teleost species especially with Danio rerio and Carassius auratus Multialignments of the four Claudin amino acid sequences have seen the two conserved cysteines existing in the first extracellular loop of Claudin15a Claudinb and Claudinc and the sequence diversity of the four Claudins mainly lies within the Cterminal tails which usually end with the YV motif except the FV motif in Claudinb Tissue distributions of the four Claudins were measured by applying quantitative realtime PCR technique Results showed that Claudin3 was mainly expressed in liver and middle intestine and Claudinb was ubiquitously expressed with a higher expression in middle intestine while Claudin15a and Claudinc were mainly expressed in middle intestine Our study revealed the existence of the TJ in the middle intestinal and obtained the genetic information of Claudin3 Claudin15a Claudinb and Claudinc of grass carp aiming to found the molecular biology basis for the further study of the intestinal barrier function of grass carp

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