Journal Title
Title of Journal: Fish Physiol Biochem
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Abbravation: Fish Physiology and Biochemistry
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Publisher
Springer Netherlands
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Authors: Daniela Volcan Almeida Bruna Félix da Silva Nornberg Laura A Geracitano Daniela Martí Barros José Maria Monserrat Luis Fernando Marins
Publish Date: 2010/09/01
Volume: 36, Issue: 3, Pages: 347-353
Abstract
We have evaluated the homology of the electrophileresponsive element EpRE core sequence a binding site for the Nrf2 transcription factor in the proximal promoters of the mouse and zebrafish glutathioneStransferase gst glutamate cysteine ligase catalytic subunit gclc and heat shock protein 70 hsp70 genes The EpRE sites identified for both species in the three analyzed genes showed a high similarity with the putative EpRE core sequence We also produced a transgenic zebrafish model carrying a transgene comprised of the luciferase luc reporter gene under transcriptional control of a mouse EpRE sequence This transgenic model was exposed to copper sulfate and the reporter gene was significantly activated The endogenous gst gclc and hsp70 zebrafish genes were analyzed in the EpRELuc transgenic zebrafish and showed an expression pattern similar to that of the reporter transgene used Our results demonstrate that EpRE is conserved between mouse and zebrafish for detoxificationrelated genes and that the development of genetically modified models using this responsive element to drive the expression of reporter genes can be an important tool in understanding the action mechanism of aquatic pollutantsThe authors would like to thank Maíra Proietti for valuable corrections of the manuscript This work was supported by FAPERGS Proc No 0412405 and CNPq/CTHIDRO Proc No 556022/20068 DV Almeida and LA Geracitano are postgraduate student and postdoctoral fellows respectively financed by CAPES JM Monserrat and D Martí Barros are research fellows from CNPq
Keywords:
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- Observation of the middle intestinal tight junction structure, cloning and studying tissue distribution of the four Claudin genes of the grass carp ( Ctenopharyngodon idellus)
- Spatial learning-induced egr - 1 expression in telencephalon of gold fish Carassius auratus
- Cloning and characterization of the HSP90 beta gene from Tanichthys albonubes Lin (Cyprinidae): effect of copper and cadmium exposure
- Protein and energy utilization and the requirements for maintenance in juvenile mulloway ( Argyrosomus japonicus )
- Evoked potentials in the Atlantic cod following putatively innocuous and putatively noxious electrical stimulation: a minimally invasive approach
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- Antioxidative role of selenium against the toxic effect of heavy metals (Cd +2 , Cr +3 ) on liver of rainbow trout ( Oncorhynchus mykiss Walbaum 1792)
- Molecular cloning, characterization, and expression analysis of luteinizing hormone receptor gene in turbot ( Scophthalmus maximus )
- Identification of Stress Sensitive Genes in Hyperthermic Atlantic Salmon ( Salmo salar ) Embryos by RAP-PCR
- Nitrogenous and phosphorus excretions in juvenile silver catfish ( Rhamdia quelen ) exposed to different water hardness, humic acid, and pH levels
- Fish condensate as effective replacer of fish meal protein in diet for striped snakehead, Channa striata (Bloch)
- The effects of cadmium and copper on embryonic and larval development of ide Leuciscus idus L.
- Substrate specificity of brain acetylcholinesterase and its sensitivity to carbamate insecticides in Carassius auratus
- Unmethylated CpG oligodeoxynucleotides activate head kidney leukocytes of Atlantic cod, Gadus morhua
- Peptide mapping of polymorphic myosin heavy chain isoforms in different muscle types of some freshwater teleosts
- Universal antisera for immunocytochemical identification of two different gonadotrophs in acanthopterygian fishes
- The isoelectric point, a key to understanding a variety of biochemical problems: a minireview
- Adaptive alterations on gill Na + , K + -ATPase activity and mitochondrion-rich cells of juvenile Acipenser sinensis acclimated to brackish water
- Polymorphisms and DNA methylation level in the CpG site of the GHR1 gene associated with mRNA expression, growth traits and hormone level of half-smooth tongue sole ( Cynoglossus semilaevis )
- Stress-induced inhibition of recruitment of ovarian follicles for vitellogenic growth and interruption of spawning cycle in the fish Oreochromis mossambicus
- Effects of dietary thiamin on the physiological status of the grouper Epinephelus coioides
- Spectral sensitivity of juvenile chub mackerel ( Scomber japonicus ) in visible and ultraviolet light
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