Journal Title
Title of Journal: Fish Physiol Biochem
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Abbravation: Fish Physiology and Biochemistry
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Publisher
Springer Netherlands
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Authors: Jin Bai Weida Gong Cuili Wang Yida Gao Wanshu Hong Shi Xi Chen
Publish Date: 2015/12/30
Volume: 42, Issue: 3, Pages: 947-954
Abstract
In vertebrates the aromatase coded by the cyp19a1a gene can catalyze the conversion from androgens to estrogens Thus the regulatory mechanisms of cyp19a1a gene expression are a critical research field in reproductive endocrinology In this study we use zebrafish as a model to study the dynamic methylation levels of the cyp19a1a gene core promoter during zebrafish ovarian folliculogenesis The results show that there is an apparent fluctuation of the methylation levels of zebrafish cyp19a1a core promoter Moreover the methylation levels are inversely correlated with the expression levels of cyp19a1a transcripts when the ovarian follicles develop from PV into the MV stage Also the CpG dinucleotides which are close to the transcriptional starting site may have provided a significant blocking effect on inhibiting the transcriptional function of RNA polymerase II Taken together the results from the present study strongly suggest that DNA methylation was one of mechanisms that are involved in the regulation of cyp19a1a gene expression during folliculogenesis This methylation mechanism modifying transcriptional process accompanied with zebrafish ovarian folliculogenesis might also shed new light on the regulation of cyp19a1a expression during the ovarian developmental stage in other vertebratesThis work was supported in part by the Marine Science Base Project for Scientific Research Training and Capacity Enhancement—Xiamen University the National Natural Science Foundation of China Nos J1210050 and 31201977 the Natural Science Foundation of Fujian Province No 2013J05029 and Xiamen Southern Oceanographic Center No 14GZY019NF19 Professor John Hodgkiss of The University of Hong Kong is thanked for his help with the English
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- Spatial learning-induced egr - 1 expression in telencephalon of gold fish Carassius auratus
- Cloning and characterization of the HSP90 beta gene from Tanichthys albonubes Lin (Cyprinidae): effect of copper and cadmium exposure
- Protein and energy utilization and the requirements for maintenance in juvenile mulloway ( Argyrosomus japonicus )
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- Molecular cloning, characterization, and expression analysis of luteinizing hormone receptor gene in turbot ( Scophthalmus maximus )
- Identification of Stress Sensitive Genes in Hyperthermic Atlantic Salmon ( Salmo salar ) Embryos by RAP-PCR
- Nitrogenous and phosphorus excretions in juvenile silver catfish ( Rhamdia quelen ) exposed to different water hardness, humic acid, and pH levels
- Fish condensate as effective replacer of fish meal protein in diet for striped snakehead, Channa striata (Bloch)
- The effects of cadmium and copper on embryonic and larval development of ide Leuciscus idus L.
- Induction of phase II enzymes and hsp70 genes by copper sulfate through the electrophile-responsive element ( EpRE ): insights obtained from a transgenic zebrafish model carrying an orthologous EpRE sequence of mammalian origin
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- Peptide mapping of polymorphic myosin heavy chain isoforms in different muscle types of some freshwater teleosts
- Universal antisera for immunocytochemical identification of two different gonadotrophs in acanthopterygian fishes
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- Adaptive alterations on gill Na + , K + -ATPase activity and mitochondrion-rich cells of juvenile Acipenser sinensis acclimated to brackish water
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- Stress-induced inhibition of recruitment of ovarian follicles for vitellogenic growth and interruption of spawning cycle in the fish Oreochromis mossambicus
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