Journal Title
Title of Journal: Fish Physiol Biochem
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Abbravation: Fish Physiology and Biochemistry
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Publisher
Springer Netherlands
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Authors: Haichao Liu Huihui Chen Jing Jing Xufa Ma
Publish Date: 2011/09/14
Volume: 38, Issue: 3, Pages: 745-756
Abstract
Heat shock protein 90 HSP90 is a highly conserved molecular chaperone important in the maturation of a broad spectrum of proteins In order to evaluate the effect of copper Cu2+ and cadmium Cd2+ on the expression of HSP90 from Tanichthys albonubes designated TaHSP90 the fulllength complementary DNA cDNA of TaHSP90 was cloned using reverse transcription PCR and rapid amplification of cDNA ends RACE techniques A 2687bp sequence was sequenced and consisted of an open reading frame ORF of 2181 bp encoding a polypeptide of 727 amino acids with five HSP90 family signatures Homologous analysis revealed that TaHSP90 gene shared high similarity with other known HSP90 genes and belonged to HSP90β subtype Fluorescent realtime quantitative PCR was used to examine the expression pattern of TaHSP90β mRNA in different tissues liver muscle gill fin eye ovary intestine and brain and the result indicated that TaHSP90β was widely expressed in all examined tissues at different levels Sensitivity of TaHSP90β to copper and cadmium was examined by exposing fish to different concentrations of Cu2+ 0 1350 and 2700 μg/L and Cd2+ 0 115 231 mg/L for 24 48 72 and 96 h respectively The copper treatment induced TaHSP90β expression slight increase only at 24 and 48 h while cadmium treatment caused slight downregulation of TaHSP90β only 72 and 96 h Our data suggest that the cloning and expression analysis of T albonubes HSP90β gene provided useful molecular information of T albonubes responses in stress conditions and potential ways to monitor the chronic stressors in T albonubes culture environmentsThis research was supported by National Major Science and Technology Projects on Management and Control of Water Pollution in China grants 2008ZX07211007 We are grateful of Dr Xichang Tan of Pearl River Research Institute Chinese Academy of Fisheries Science for his aid in collecting experiment fish We thank Dr Laura Tiu of Ohio State University USA and Dr Hongmei Cai of College of Resources and Environment Huazhong Agricultural University China for their valuable language helps in preparing the paper
Keywords:
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