Journal Title
Title of Journal: Fish Physiol Biochem
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Abbravation: Fish Physiology and Biochemistry
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Publisher
Springer Netherlands
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Authors: F M A H Schuurmans Stekhoven M H A G Gorissen G Flik
Publish Date: 2007/08/09
Volume: 34, Issue: 1, Pages: 1-8
Abstract
We address the importance of the isoelectric point IEP of proteins and membrane components such as phospholipids for our understanding and interpretation of isoforms and opposite charge interactions in the formation of complexes Five examples drawn from the literature are newly approached from the IEP point of view to clarify general principlesThe isoelectric point IEP of a protein is generally considered to be a mere physicochemical property that results from the summed ambivalent charge properties of its amino acid constituents In fact the IEP values of proteins are often not even recorded in data books despite the primary structure of the protein being fully known We show here that the IEP is a powerful tool to predict and understand interactions between proteins proteins and membranes phospholipids or to determine the presence of protein isoformsThis equation is identical to the formulation given by Sillero and Ribeiro 1989 mathopSigmalimitsp i=1frac11+10rm pHpKPi mathopSigmalimitsn i=1frac11+10rm pKNipH = 0 with pKPi being equivalent to −10log KDbj and pKNi equivalent to −10logKDai We use Eq 3 and determine the IEP graphically from the intersection with the axis of zero charge difference in the Δcharge versus pH chart The EMBL website gateway to IEP service http//wwwemblheidelbergde/cgi/piwrapperpl gives similar results with a maximal deviation of only a few percentage On the other hand the programme WinPep http//wwwipwagrlethzch/∼lhennig/winpephtml gives totally different – often too low – figures and has been avoided here for the calculation of the IEP However this programme is reliable for the calculation of the hydropathy plot according to the procedure of Kyte and Doolittle 1982 which was applied to determine the membranetraversing moieties of the proteins discussed
Keywords:
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- Molecular cloning, characterization, and expression analysis of luteinizing hormone receptor gene in turbot ( Scophthalmus maximus )
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