Authors: Itsuki Watanabe Toshihide Nakamura Jun Shima
Publish Date: 2008/07/03
Volume: 35, Issue: 10, Pages: 1117-
Abstract
Contamination of Lactobacillus sp in the fermentation broth of bioethanol production decreases ethanol production efficiency Although the addition of lactate to the broth can effectively inhibit the growth of Lactobacillus sp it also greatly reduces the fermentation ability of Saccharomyces cerevisiae To overcome this conflict lactatetolerant yeast strains were screened Candida glabrata strain NFRI 3164 was found to exhibit both higher levels of lactate tolerance and fermentation ability Cocultivation of C glabrata was performed with Lactobacillus brevis and Lb fermentum which were reported as major contaminating bacteria during bioethanol production in culture medium containing 2 lactate Under these culture conditions the growth of Lactobacillus strains was greatly inhibited but the ethanol production of C glabrata was not significantly affected Our data show the possibility of designing an effective fuel ethanol production process that eliminates contamination by Lactobacillus strains through the combined use of lactate addition and C glabrataThis work was supported by the project for the development of biomass utilization technologies for revitalizing rural areas from the Ministry of Agriculture Forestry and Fisheries MAFF of Japan We thank S MizukamiMurata and Y Sakayori National Food Research Institute for their technical assistance in this study
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