Authors: YingXiu Cao Hua Lu Bin Qiao Yao Chen YingJin Yuan
Publish Date: 2012/10/09
Volume: 40, Issue: 1, Pages: 95-103
Abstract
Cephalosporin C CPC is the precursor of a class of antibiotics that were more effective than traditional penicillins CPC production is performed mainly through fermentation by Acremonium chrysogenum whose secondary metabolism was sensitive to the environmental changes In the present work secondary metabolites were measured by ionpair reversedphase liquid chromatography tandemed with hybrid quadrupole timeofflight mass spectrometry and the disparity of them from two scales of CPC fermentations pilot and industrial and also two different posttreatment processes oxalic acid and formaldehyde added and control were investigated When fermentation size was enlarged from pilot scale 50 l to industrial scale 156000 l the remarkable disparities of concentrations and changing trends of the secondary metabolites in A chrysogenum were observed which indicated that the productivity of CPC biosynthesis was higher in the large scale of fermentation Three environmental factors were measured and the potential reasons that might cause the differences were analyzed In the posttreatment process after industrial fermentation the changes of these secondary metabolites in the tank where oxalic acid and formaldehyde were added were much less than the control tank where none was added This indicated that the quality of the final product was more stable after the oxalic acid and formaldehyde were added in the posttreatment process These findings provided new insight into industrial CPC productionThe authors are grateful for the financial support from the National Hightech RD Program 863 Program 2012AA021204 2012AA02A701 National Basic Research Program of China 973 Program 2013CB733601 and the National Natural Science Foundation of China Major International Joint Research Project 21020102040
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