Authors: Ting Guo Yan Tang Qiuyan Zhang Tengfei Du Dafeng Liang Min Jiang Pingkai Ouyang
Publish Date: 2011/07/26
Volume: 39, Issue: 3, Pages: 401-407
Abstract
Clostridium beijerinckii mutant strain IB4 which has a high level of inhibitor tolerance was screened by lowenergy ion implantation and used for butanol fermentation from a nondetoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid SAHHC Evaluation of toxicity showed C beijerinckii IB4 had a higher level of tolerance than parent strain C beijerinckii NCIMB 8052 for five out of six phenolic compounds tested the exception was vanillin Using glucose as carbon source C beijerinckii IB4 produced 91 g l−1 of butanol with an acetone/butanol/ethanol ABE yield of 041 g g−1 When nondetoxified SAHHC was used as carbon source C beijerinckii NCIMB 8052 grew well but ABE production was inhibited By contrast C beijerinckii IB4 produced 95 g l−1 of ABE with a yield of 034 g g−1 including 22 g l−1 acetone 68 g l−1 butanol and 05 g l−1 ethanol The remarkable fermentation and inhibitor tolerance of C beijerinckii IB4 appears promising for ABE production from lignocellulosic materialsThis work was supported by the “973” program of China grant no 2011CB707405 the public benefit research foundation of CAF 201004001 the State Key Laboratory of MaterialsOriented Chemical Engineering Foundation of Nanjing University of Technology ZK201001 a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions the “Qinglan project” of Jiangsu Province and “The six talent summit” of Jiangsu Province grant no 06A047
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