Authors: C A Müller A M Weingartner A Dennig A J Ruff H Gröger Ulrich Schwaneberg
Publish Date: 2016/10/22
Volume: 43, Issue: 12, Pages: 1641-1646
Abstract
A novel whole cell cascade for double oxidation of cyclooctane to cyclooctanone was developed The onepot oxidation cascade requires only a minimum of reaction components resting E coli cells in aqueous buffered medium =catalyst the target substrate and oxygen as environmental friendly oxidant Conversion of cyclooctane was catalysed with high efficiency 50 yield and excellent selectivity 94 to cyclooctanone The reported oxidation cascade represents a novel whole cell system for double oxidation of nonactivated alkanes including an integrated cofactor regeneration Notably two alcohol dehydrogenases from Lactobacillus brevis and from Rhodococcus erythropolis with opposite cofactor selectivities and one monooxygenase P450 BM3 were produced in a coexpression system in one single host The system represents the most efficient route with a TTN of up to 24363 being a promising process in terms of sustainability as wellWe would like to thank Evonik Industries and the German Federal Ministry of Education and Research BMBF funding number 0316044A We also thank Till Winkler and Prof Dr Werner Hummel FZ Jülich for providing the ADH enzymes as well as Dr Steffen Schaffer Dr Oliver Thum Dr Markus Pötter and Michaela Hauberg Evonik Industries Marl Germany for helpful discussions
Keywords: