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Title of Journal: J Ind Microbiol Biotechnol

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Abbravation: Journal of Industrial Microbiology & Biotechnology

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Springer-Verlag

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DOI

10.1016/0304-8853(94)90040-x

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ISSN

1476-5535

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Fungal βglucosidase expression in Emphasis Type=

Authors: A P Njokweni S H Rose W H van Zyl
Publish Date: 2012/06/16
Volume: 39, Issue: 10, Pages: 1445-1452
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Abstract

Recombinant Saccharomyces cerevisiae strains expressing βglucosidases from Thermoascus aurantiacus Tabgl1 and Phanerochaete chrysosporium PcbglB and Pccbgl1 were constructed and compared to S cerevisiae Y294SFI previously identified as the best βglucosidaseproducing strain The PcbglB was also intracellularly expressed in combination with the lac12 lactose permease of Kluyveromyces lactis in S cerevisiae Y294PcbglB + Lac12 The recombinant extracellular βglucosidases indicated maximum activity in the pH range 4–5 and temperature optima varying from 50 to 75 °C The S cerevisiae Y294Pccbgl1 strain performed best under aerobic and anaerobic conditions producing 26 times more βglucosidase activity than S cerevisiae Y294SFI and an ethanol concentration of 48 g l−1 after 24 h of cultivation on cellobiose as sole carbohydrate source S cerevisiae Y294Tabgl1 was unable to grow on cellobiose liquid medium whereas S cerevisiae Y294PcbglB + Lac12 exhibited limited growth

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