Authors: Weihong Zhong Jianjun Fang Huagui Liu Xin Wang
Publish Date: 2009/02/17
Volume: 36, Issue: 5, Pages: 687-693
Abstract
The use of coenzyme Q10 CoQ10 as a complementary therapy in heart failure will increase in proportion to the growth of the ageing population and the expansion of statins consumption Economical production of CoQ10 by microbes will become more important due to the growing demands of the pharmaceutical industry Process simplification and integration might be one desirable pathway for economic production of CoQ10 by microbial fermentation In this report the effect of a coupled fermentation–extraction process on CoQ10 production by newly isolated Sphingomonas sp ZUTEO3 was evaluated It was found that the CoQ10 yield of the coupled process was significantly higher than that of the traditional process As optimal conditions in our experiment 2 soybean oil was added to the original culture to enhance cell membrane permeability and 50 mL hexane was added to the 30 h culture as an extracting solvent for the subsequent coupled fermentation–extraction process The maximal yield of CoQ10 reached 432 mg/L and 325 mg/g dry cell weight after 38 h of total fermentation period The coupled process represents one potential pathway for CoQ10 production with even higher yield and lower cost This is the first report of CoQ10 production by Sphingomonas sp using a coupled fermentation–extraction process
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