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Title of Journal: J Ind Microbiol Biotechnol

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Abbravation: Journal of Industrial Microbiology & Biotechnology

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Springer Berlin Heidelberg

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DOI

10.1007/s12133-008-0041-z

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1476-5535

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Emphasis Type="Italic"SlnM/Emphasis gene overe

Authors: Huiling Wu Weicheng Liu Dan Dong Jinjin Li Dianpeng Zhang Caige Lu
Publish Date: 2013/10/31
Volume: 41, Issue: 1, Pages: 163-172
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Abstract

Natamycin is an important polyene macrolide antifungal agent produced by several Streptomyces strains and is widely used as a food preservative and fungicide in food medicinal and veterinary products In order to increase the yield of natamycin this study aimed at cloning and overexpressing a natamycinpositive regulator slnM2 with different promoters in the newly isolated strain Streptomyces lydicus A02 which is capable of producing natamycin The slnM gene in S lydicus is highly similar to gene pimM scnRII the pathwayspecific positive regulator of natamycin biosynthesis in S natalensis and S chattanoogensis which are PASLuxR regulators Three engineered strains of S lydicus AM01 AM02 and AM03 were generated by inserting an additional copy of slnM2 with an ermEp promoter inserting an additional copy of slnM2 with dual promoters ermEp and its own promoter and inserting an additional copy of slnM2 with its own promoter respectively No obvious changes in growth were observed between the engineered and wildtype strains However natamycin production in the engineered strains was significantly enhanced by 24fold in strain AM01 30fold in strain AM02 and 19fold in strain AM03 when compared to the strain A02 in YEME medium without sucrose These results indicated that the ermEp promoter was more active than the native promoter of slnM2 Overall dual promoters displayed the highest transcription of biosynthetic genes and yield of natamycinThis work was supported by the Science and Technology Plan Project of Beijing no Z121100001212002 Special Research Fund for AgroScientific Research in the Public Interest from the Chinese Agriculture Ministry no 20090304907 and Science Research Innovation Fund from Institute of Plant and Environment Protection Beijing Academy of Agriculture and Forestry Sciences no CCJJZDXM2012012 The authors thank Dr Davide Spadaro Assistant Professor of DISAFA University of Torino for technical reading


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  2. Wood pulp as an immobilization matrix for the continuous production of isopropanol and butanol
  3. Persistence of Methanosaeta populations in anaerobic digestion during process instability
  4. Xylitol production from DEO hydrolysate of corn stover by Pichia stipitis YS-30
  5. Biorefining of wood: combined production of ethanol and xylanase from waste fiber sludge
  6. Do we need new antibiotics? The search for new targets and new compounds
  7. Sporicidal efficacy of pH-adjusted bleach for control of bioburden on production facility surfaces
  8. A novel methodology independent of fermentation rate for assessment of the fructophilic character of wine yeast strains
  9. Engineered glucose isomerase from Streptomyces sp. SK is resistant to Ca 2+ inhibition and Co 2+ independent
  10. Universal species concept: pipe dream or a step toward unifying biology?
  11. A strategy to prevent the occurrence of Lactobacillus strains using lactate-tolerant yeast Candida glabrata in bioethanol production
  12. Recent biotechnological progress in enzymatic synthesis of glycosides
  13. Enhanced production of poly(3-hydroxybutyrate- co -4-hydroxybutyrate) copolymer with manipulated variables and its properties
  14. Comparison of the secondary metabolites in two scales of cephalosporin C (CPC) fermentation and two different post-treatment processes
  15. Clostridium beijerinckii mutant with high inhibitor tolerance obtained by low-energy ion implantation
  16. Enhanced production of CoQ 10 by newly isolated Sphingomonas sp. ZUTEO3 with a coupled fermentation–extraction process
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  18. Ivan Málek and the Institute of Microbiology
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  20. A whole cell biocatalyst for double oxidation of cyclooctane
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  22. Use of interdelta polymorphisms of Saccharomyces cerevisiae strains to monitor population evolution during wine fermentation
  23. Antimicrobial susceptibility of Lactobacillus species isolated from commercial ethanol plants
  24. Distribution of live and dead cells in pellets of an actinomycete Amycolatopsis balhimycina and its correlation with balhimycin productivity
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  29. Development of a biochip using antibody-coated gold nanoparticles to detect specific bioparticles
  30. Significance of oxygen carriers and role of liquid paraffin in improving validamycin A production

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