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Title of Journal: J Ind Microbiol Biotechnol

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Abbravation: Journal of Industrial Microbiology & Biotechnology

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Springer-Verlag

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DOI

10.1016/0038-1101(83)90054-0

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1476-5535

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Production of a novel extracellular acidic lipase

Authors: K Ramani Evvie Chockalingam G Sekaran
Publish Date: 2010/03/04
Volume: 37, Issue: 5, Pages: 531-535
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Abstract

An isolate exhibiting high extracellular lipolytic activity was identified as Pseudomonas gessardii by 16S rDNA gene sequence analysis The slaughterhouse waste goat tallow was used as a lipid substrate for the production of acidic lipase by P gessardii The maximum lipase activity of 156 U/ml was observed at an acidic pH of 35 and at 031 g substrate concentration The purification steps resulted in the isolation of acidic lipase with a specific activity of 1473 U/mg and a molecular weight of 94 kDa One interesting feature of this purified lipase is its stability at highly acidic pH ranging from 20 to 55 with a high molecular weight The amino acid composition was determined using HPLC This acidic lipase has potential applications in the medicinal field as a substitute for pancreatic lipases for enzyme therapy oleochemical and in biotechnological industriesThe author K Ramani is thankful to the Council of Scientific and Industrial Research CSIR New Delhi India and the Central Leather Research Institute CLRI India for awarding a research fellowship and providing the facilities needed to carry out this work

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