Authors: Mustafa Tunalı Tamer Ataoğlu Ilhami Çelik
Publish Date: 2013/12/07
Volume: 18, Issue: 7, Pages: 1825-1833
Abstract
Diabetes mellitus DM is wellestablished risk factor for periodontal disease DM can also lead to changes in the number of apoptotic cells in periodontal tissues The goal of this study was to evaluate apoptosis depending on DM in healthy and diseased periodontal soft tissuesA total of 43 adult male Sprague–Dawley rats were used in this study Experimental periodontitis was created by placing silk ligatures around the cervices of the first mandibular molars Experimental diabetes was induced by intraperitoneal injection of the diabetogenic agent streptozotocin STZ Following the induction of both experimental diseases the animals were divided into four groups 1 The healthy group H n = 10 2 The diabetes group D n = 10 3 The periodontitis group P n = 11 and 4 The diabetes and periodontitis group DP n = 12 Apoptotic cells were determined by immunohistochemistry and the frequency of apoptotic cells was evaluated by apoptotic index scoreIt was observed that there was less apoptosis in both the epithelial and gingival connective tissue cells of healthy diabetic tissues than in healthy tissues without diabetes When periodontal disease existed apoptosis increased in both the epithelial and gingival connective tissues of diabetic and nondiabetic animals“I affirm that we have no financial affiliation eg employment direct payment stock holdings retainers consultantships patent licensing arrangements or honoraria or involvement with any commercial organization with direct financial interest in the subject or materials discussed in this manuscript nor have any such arrangements existed in the past 3 years Any other potential conflict of interest is disclosed”
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