GAL3ST2 from mammary gland epithelial cells affect

Authors: L N Guerra C Suarez D Soto A Schiappacasse D Sapochnik P Sacca G PiwienPilipuk B Peral J C Calvo

Publish Date: 2014/12/23

Volume: 17, Issue: 7, Pages: 511-520

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Abstract

In the mammary gland the involution that occurs when lactation ends is an important period for cancer development We have previously demonstrated stromal–epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity Creydt et al Clin Transl Oncol 15124–131 2013 Here we evaluated the effects of conditioned medium of breast epithelial mammary cells on stromal cellsWe described for the first time that breast epithelial mammary cells could produce the enzyme galactose 3Osulfotransferase 2 GAL3ST2 Importantly GAL3ST2 is present in NMMuMG and two human breast cancer cell lines and it is more strongly expressed in more metastatic tumors When 3T3L1 preadipocyte differentiation was triggered in the presence of conditioned medium from NMuMG or GAL3ST2 triglyceride accumulation was decreased by 40  and C/EBPβ expression by 80  in adipocytes In addition the expression of FABP4 aP2 another marker of adipocyte differentiation was inhibited by 40  in GAL3ST2treated cellsTaken together these results suggest that GAL3ST2 would interfere with normal differentiation of 3T3L1 preadipocytes raising the possibility that it may affect normal differentiation of stromal preadipocytes and be a link to tumor metastatic capacityWe thank Dr Honke for his contribution to this work This study was supported by Grant UBACYT 20020110200187 Universidad de Buenos Aires Argentina OAT 42/13 Universidad de Buenos Aires Argentina and Grant SAF201233014 Ministerio de Ciencia e Innovación Tecnológica España

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