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Title of Journal: Monatsh Chem

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Abbravation: Monatshefte für Chemie - Chemical Monthly

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Springer Vienna

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DOI

10.1016/0008-6223(68)90373-4

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1434-4475

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Switching central trinucleotide sequences of DNA h

Authors: Iveta Triskova Libor Gurecky Libuse Trnkova
Publish Date: 2016/02/22
Volume: 147, Issue: 5, Pages: 857-864
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Abstract

The article aims to present an electrochemical investigation of DNA heptamers dGCNNNGC with a different central trinucleotide sequence NNN = GAA AAA CCC and GGG where A C and G is adenine cytosine and guanine respectively In phosphateacetate buffer at pH 58 all heptamers were adsorbed on a hanging mercury electrode and these adsorbed heptamers underwent reduction and oxidation The voltammetric reduction peaks of A and C moiety and oxidation peaks of G moiety at different concentrations 025–10 µmol dm−3 were analyzed It was found that the changes in the central trinucleotide sequence of the heptamers result in altered adsorption ability at a charged phase interface An electron transfer process in an adsorptive state was evidenced by the elimination voltammetric procedure For the evaluation of adsorption efficiency of the heptamers the Langmuir adsorption model was applied and the adsorption coefficient b was calculated


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