Authors: D Bentrop J Kohr H Engelhardt
Publish Date: 1991/08/01
Volume: 32, Issue: 3-4, Pages: 171-178
Abstract
Thermal polymerization of the Ncarboxyanhydride of glutamic acid5methylester was used to coat microparticulate silica and the surface of capillaries with polymethylglutamate PMG By increasing the thickness of the PMG layer the hydrophobicity of the stationary phases could be increased However total shielding of surface silanols was not achieved The PMGcoated silicas for HPLC showed unexpected and strange behavior in the chromatography of proteins Nevertheless capillaries coated with PMG proved to be excellent for highly efficient CE separations of proteins at medium pH values
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