HPLC Method for the Dosage of Paclitaxel in Copaib

Authors: Francisco Humberto Xavier Junior Claire Gueutin Andreza Rochelle do Vale Morais Everton do Nascimento Alencar Eryvaldo Sócrates Tabosa do Egito Christine Vauthier

Publish Date: 2016/02/27

Volume: 79, Issue: 7-8, Pages: 405-412

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Abstract

A highly sensitive simple rapid innovative and economic method has been developed and validated for the quantification of paclitaxel in a complex plant matrix copaiba oil by HPLC method Chromatographic separation was performed by Uptisphere Strategy C18 column and the detection was performed at 228 nm No interfering peaks from highly complex matrices were observed during the paclitaxel elution Standard curves of paclitaxel containing 10 mg of copaiba resin oil were linear over the concentration range from 200 to 1700 ng mL−1 with a determination coefficient of 0999 and lower regression residues with a homoscedastic dispersion The lower quantification and detection limits were 338 and 101 ng mL−1 respectively The accuracy and precision determinations were less or equal to 060 and 203  respectively The method developed was successfully applied to the evaluation of paclitaxel in copaiba resin and essential oils by solubility and partition coefficient studies Paclitaxel showed a lipophilic characteristic log P  1 and a higher solubility in copaiba oils In conclusion the method showed sensitivity linearity precision accuracy necessary specificity and robustness for a reliable separation identification and quantification of paclitaxel in complex plant matrix This new analytical method can be therefore also applied to quantify paclitaxel in drug delivery systems in which copaiba resin or essential oils are associated such as lipid and polymer systems

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