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Title of Journal: Chromatographia

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Abbravation: Chromatographia

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Vieweg Verlag

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DOI

10.1002/9781118996096.ch14

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1612-1112

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Evaluation of Nonionic Block Polymer Surfactants i

Authors: Hongbo Ma Yufeng Hu Futong Yu Xueqin Ren Fusuo Zhang
Publish Date: 2011/02/26
Volume: 73, Issue: 7-8, Pages: 681-690
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Abstract

In the present work six nonionic block copolymer surfactants consisting of polyethylene glycol PEG polyethylene PE and polypropylene glycol PPG were exploited to extract total proteins from maize Zea mays L roots within a water–organic solvent system After the treatment proteins were partitioned into aqueous phase interphase and organic phase The total yield was increased by up to 30 after adding PEPEG block polymer surfactant compared with control Twodimensional electrophoresis 2DE was further used to evaluate proteins in water phase and interphase the results revealed that the spot numbers and density were all improved and wellfocused electrophoretic patterns were achieved with higher density and without excess Joule heating problem after adding nonionic block polymer surfactants Among the six investigated block polymers PEPEG extracted the largest amount of proteinsThis work was supported by Chinese National Scientific Foundation 20875100 Ministry of Education of China National Key Technology R D Program 2006BAD10B02 2008BADA4B04 by Ministry of Science Technology of China the Innovative Group Grant of NSFC 30821003 funded by Ministry of Education of China

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