Ion Chromatographic Analysis of Monosaccharides an

Authors: Warangkana Suksom Wannilak Wannachai Suthasinee Boonchiangma Saksit Chanthai Supalax Srijaranai

Publish Date: 2015/02/19

Volume: 78, Issue: 13-14, Pages: 873-879

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Abstract

A simple and effective method using ion chromatography was developed for the simultaneous determination of five monosaccharides arabinose glucose fructose xylose and ribose and two disaccharides sucrose and lactose in raw sugar samples The separation was performed on a CarboPac PA 10 column using the gradient elution of sodium hydroxide and water as the mobile phase Monosaccharides and disaccharides were detected by an integrated pulsed amperometric detection IPAD using gold working electrode Acid hydrolysis was used for sample preparation before the analysis of glucose and fructose All the studied sugars showed good linear ranges within 05–100 µg mL−1 with the correlation coefficients higher than 0997 The limits of detection were all less than 05 µg mL−1 The RSDs of the method were less than 10  The recoveries of the sugars that spiked in raw sugar samples ranged from 961 to 1024  The method was successfully applied for the analysis of sugars in raw sugar samples Sucrose is the major constituent found in the samples at 97 Financial support from the Center of Excellence for Innovation in Chemistry PERCHCIC Commission on Higher Education Ministry of Education Thailand is gratefully acknowledged Raw sugar samples from Mitr Phol Factory Khon Kaen Thailand are acknowledged

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