Comparison of Two StabilityIndicating Chromatogra

Authors: Ali M Yehia Iman Sami Safa’a M Riad Yasser S ElSaharty

Publish Date: 2016/11/29

Volume: 80, Issue: 1, Pages: 99-107

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Abstract

Mirabegron is a novel β3adrenoceptor agonist containing an amide group It was subjected to stress conditions of acidic and alkaline hydrolyses The hydrolytic degradation product was isolated and its structure was confirmed using mass and IR spectrometry Two stabilityindicating chromatographic methods have been proposed for the determination of mirabegron TLC method was applied using silica gel as stationary phase and chloroform–methanol–ammonia 901001 by volume as the mobile phase and chromatograms were scanned at 250 nm Accurate determination of the drug was achieved over the concentration range of 2–12 μg per band In addition an isocratic HPLC method was developed on Agilent C18 column 150 mm × 45 mm ID particle size 5 µm using ethanolphosphate buffer pH 25 3070 by volume as a mobile phase with flow rate of 1 mL min−1The intact drug was detected at 250 nm with running time less than 5 min Mirabegron was determined accurately in a concentration range of 1–25 µg mL−1 The proposed chromatographic methods were applied successfully for the assay of mirabegron in pharmaceutical dosage form and both methods were validated as per the International Conference on Harmonization guidelines and statistically compared with a reported gradient HPLC method

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