Rapid Screening of Volatile IonPair Reagents Usin

Authors: Girish R Deshpande Amrendra K Roy N Someswara Rao B Mallikarjuna Rao J Rudraprasad Reddy

Publish Date: 2011/02/24

Volume: 73, Issue: 7-8, Pages: 639-648

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Abstract

As over 70 of pharmaceutical compounds are bases the analysis of these basic compounds by high performance liquid chromatography HPLC continues to be of great value and interesting Acetyl cholinesterase inhibitors AChEIs which contain the basic compounds like Rivastigmine tartrate Galantamine hydrobromide and Donepezil with different polarities were chosen for the study A rapid screening of the volatile ionpairing reagents was performed using modern techniques like ultra high performance liquid chromatography UHPLC The experiments were planned using the ‘Design of Experiments’ DoE approach to identify the LC–MS compatible ionpair reagent In this study Heptafluorobutyric acid HFBA has given a very good peak shape with tailing factor at 14 and theoretical plates up to ~5000 were observed compared to tailing factor at 19 and theoretical plates up to ~3000 with nonvolatile ionpair reagent sodium heptane sulphonate SHS Similarly retention with HFBA was optimum as ~5 min in short run time method compared to ~13 min with SHS This ionpair reagent has proved to be good replacement of sodium alkyl sulphonate modifiers HFBA can also be used for semipreparative work for isolation of impurities by just evaporating the solvents It avoids the extraction of other inorganic modifiers

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