Journal Title
Title of Journal: Antonie van Leeuwenhoek
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Abbravation: Antonie van Leeuwenhoek
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Publisher
Springer Netherlands
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Authors: Daniel J Julian Christopher J Kershaw Nigel L Brown Jon L Hobman
Publish Date: 2008/11/13
Volume: 96, Issue: 2, Pages: 149-
Abstract
Metal responsive MerR family transcriptional regulators are widespread in bacteria and activate the transcription of genes involved in metal ion detoxification efflux or homeostasis in response to the presence of cognate metal species in the cytoplasm MerR family regulators recognize and bind to dyad symmetrical DNA sequences in specific promoters that have a spacer region between the –35 and –10 sequences which is longer than the canonical 16–18 bp spacer for other σ70–dependent promoters In this study we report βgalactosidase assays of MerR familyregulated gene expression in the multiple metal resistant bacterium Cupriavidus metallidurans A series of pMU2385 reporter plasmid derivatives containing cloned MerR familyactivated promoters were used to determine metal ioninduced responses from different MerR family regulated promoters as well as regulators cloned with the cognate promoter into pMU2385 Mercuric ionresponsive MerR and lead ionresponsive PbrR activity was confirmed using this assay system as well as MerR family activator activity on heterologous promoters PcopA PcadA and Pzcc from Escherichia coli Pseudomonas aeruginosa and Bordetella pertussis respectively In C metallidurans CH34 transcription from these promoters was activated by MerR family regulators encoded on the chromosome or megaplasmids in response to copper PcopA and lead PcadA and PzccA showing that MerR family activators in C metallidurans can act on MerR family promoters from other organisms which have sequence differences to the predicted C metallidurans promotersThis work was supported by Biotechnology and Biological Sciences Research Council grant B10333 and a BBSRC studentship to DJJ The Birmingham Functional Genomics laboratory was supported by the Biotechnology and Biological Sciences Research Council Joint Infrastructure Fund grant JIF13209
Keywords:
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