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Title of Journal: Antonie van Leeuwenhoek

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Abbravation: Antonie van Leeuwenhoek

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Springer Netherlands

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DOI

10.1007/s11802-008-0294-8

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1572-9699

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The ABCtransporter AtmA is involved in nickel and

Authors: André Mikolay Dietrich H Nies
Publish Date: 2009/01/09
Volume: 96, Issue: 2, Pages: 183-
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Abstract

Cupriavidus metallidurans CH34 genome contains an ortholog of Atm1p named AtmA Rmet 0391 YP 582546 In Saccharomyces cerevisiae the ABCtype transport system Atm1p is involved in export of iron–sulfur clusters from mitochondria into the cytoplasm for assembly of cytoplasmic iron–sulfur containing proteins An ∆atmA mutant of C metallidurans was sensitive to nickel and cobalt but not iron cations AtmA increased also resistance to these cations in Escherichia coli strains that carry deletions of the genes for other nickel and cobalt transport systems In C metallidurans atmA expression was not significantly induced by nickel and cobalt but repressed by zinc AtmA was purified as a 70 kDa protein after expression in E coli ATPase activity of AtmA was stimulated by nickel and cobalt

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