Emphasis Type="Italic"Aliiglaciecola coringensis

Authors: Vasundhera Gupta Gunjan Sharma T N R Srinivas P Anil Kumar

Publish Date: 2014/09/13

Volume: 106, Issue: 6, Pages: 1097-1103

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Abstract

A Gramnegative rod shaped motile aerobic bacterium designated as strain AK49T was isolated from a water sample from a mangrove forest in Coringa village Andhra Pradesh India Strain AK49T was observed to form yellow coloured smooth circular convex colonies on marine agar with entire margins Cells of strain AK49T are 05–10 µm wide and 15–35 µm long Growth was observed at 25–37 °C optimum 30 °C 2–6  NaCl optimum 2  and pH 6–8 optimum 7 Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain AK49T is closely related to two species recently reclassified as members of the genus Aliiglaciecola Aliiglaciecola lipolytica JCM 15139T sequence similarity 9543  and Aliiglaciecola litoralis JCM 15896T sequence similarity 9691  The major cellular fatty acids of strain AK49T were found to include C160 C181ω7c and summed feature 3 C161ω7c/C150 iso2OH The polar lipid content of cell membrane was found to include phosphatidylethanolamine phosphatidylglycerol an unidentified aminolipid an unidentified lipid and an unidentified glycolipid The genomic DNA G+C content of strain AK49T was determined to be 419 mol Based on the taxonomic methods including chemotaxonomic phenotypic and phylogenetic approaches strain AK49T is described here as a novel species belonging to the genus Aliiglaciecola for which the name Aliiglaciecola coringensis sp nov is proposed The type strain of Aliiglaciecola coringensis sp nov is AK49T =MTCC 12003T = JCM19197TWe thank the Council of Scientific and Industrial Research CSIR the Directors of IMTECH Chandigarh and NIO Goa and Department of Biotechnology as well as the Government of India for financial assistance and encouragement We would like to thank Mr Deepak for his excellent help in sequencing DNA TNRS is thankful to project PSC0105 for providing facilities and funding respectively IMTECH communication number is 036/2014 and NIO contribution number is 5649

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