Authors: Thomas P West
Publish Date: 2010/01/12
Volume: 97, Issue: 3, Pages: 307-311
Abstract
Control of pyrimidine formation was examined in Pseudomonas fulva ATCC 31418 Pyrimidine supplementation lowered pyrimidine biosynthetic pathway enzyme activities in cells grown on glucose or succinate as a carbon source indicating possible repression of enzyme synthesis Pyrimidine limitation experiments were conducted using an orotidine 5′monophosphate decarboxylase mutant strain isolated in this study Compared to uracilsupplemented glucosegrown mutant cells pyrimidine limitation of this strain caused aspartate transcarbamoylase dihydroorotase dihydroorotate dehydrogenase and orotate phosphoribosyltransferase activities to increase about 6 13 3 15fold respectively which confirmed regulation of enzyme synthesis by pyrimidines At the level of enzyme activity transcarbamoylase activity in Ps fulva was strongly inhibited by pyrophosphate CTP GTP and GDP under saturating substrate concentrations
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