Rare or rarely detected Emphasis Type="Italic"C

Authors: Teeratas Kijpornyongpan M Catherine Aime

Publish Date: 2016/05/28

Volume: 109, Issue: 8, Pages: 1127-1139

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Abstract

Ceraceosorales is a monotypic order in Ustilaginomycotina Its namesake Ceraceosorus bombacis was described as a phytopathogen of Bombax ceiba in India In this study we describe Ceraceosorus guamensis sp nov collected on the South Pacific island of Guam which appears to represent the second isolation of any member of this order in over 40 years Ceraceosorus species are monokaryotic and filamentous in culture producing conidia on potato dextrose agar However both species behave yeastlike when cultured on corn meal agar The internal transcribed spacer ITS region spanning the ITS1–58S–ITS2 in both species of Ceraceosorus is highly heterogeneous containing multiple disparate copies that can vary intragenomically by up to 35  Moreover this region could not be amplified using the fungal ITS primers most frequently used for cultureindependent methods of assessing fungal biodiversity This fact combined with the extremely slow growth rates on commonly employed media may indicate that members of this lineage are potentially underdetected by current sampling methodsWe thank the Plant and Pest Diagnostic Laboratory PPDL for microplate facilities and the lab of Dr JinRong Xu for microscope facilities Dr Robert Schlub is kindly acknowledged for facilitating collecting on Guam by MCA TK study at Purdue University is funded by an Anadamahidol Foundation Scholarship Thailand Finally we would like to thank John F Klimek and all members of the Aime lab for help in culture maintenance and molecular lab support and three anonymous reviewers for helpful comments on an earlier version of this manuscript

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