Emphasis Type="Italic"Sphingobacterium pakistane

Authors: Iftikhar Ahmed Muhammad Ehsan Yeseul Sin Jayoung Paek Nauman Khalid Rifat Hayat Young H Chang

Publish Date: 2013/11/27

Volume: 105, Issue: 2, Pages: 325-333

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Abstract

The taxonomic status of a bacterium strain NCCP246T isolated from rhizosphere of Vigna mungo was determined using a polyphasic taxonomic approach The strain NCCP246T can grow at 16–37 °C optimum 32 °C at pH ranges of 6–8 optimum growth occurs at pH 7 and in 0–4  w/v NaCl Phylogenetic analysis based upon on 16S rRNA gene sequence comparison revealed that strain NCCP246T belonged to genus Sphingobacterium Strain NCCP246T showed highest similarity to the type strain of Sphingobacterium canadense CR11T 9767  and less than 97  with other species of the genus The DNA–DNA relatedness value of strain NCCP246T with S canadense CR11T and Sphingobacterium thalpophilum JCM 21153T was 55 and 444  respectively The chemotaxonomic data revealed the major menaquinone as MK7 and dominant cellular fatty acids were summed feature 3 C161 ω7c/C161 ω6c 3707  isoC150 2803  C160 1185  C170 cyclo 884  and C140 242  The G+C content of the strain was 392 mol On the basis of DNA–DNA hybridization phylogenetic analyses physiological and biochemical data strain NCCP246T can be differentiated from the validly named members of genus Sphingobacterium and thus represents as a new species for which the name Sphingobacterium pakistanensis sp nov is proposed with the type strain NCCP246T = JCM18974 T = KCTC 23914TWe are thankful to Professor Dr George Lazarovits from Southern Crop Protection and Food Research Centre Canada and Dr Moriya Ohkuma from Japan Collection of Microorganisms JCM Riken BioResource Centre Tsukuba Japan for providing the reference strains Sphingobacterium canadense CR11T and S thalpophilum JCM 21153T respectively for these experiments This work was partially supported by PSDP Project “Research for Agricultural Development Program RADP” funded by Pakistan Agricultural Research Council PARC The partial financial support of KRIBB to the author “Muhammad Ehsan” in the training course at Korean Collection for Type Cultures Biological Resources Centre KRIBB under the umbrella of ACM forum is also gratefully acknowledged

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