Emphasis Type="Italic"Bacillus sediminis/Emphas

Authors: Zhen Yu Yueqiang Wang Dongxing Qin Guiqin Yang Shungui Zhou

Publish Date: 2013/09/14

Volume: 104, Issue: 6, Pages: 1109-1116

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Abstract

A Gramstain positive facultative anaerobic motile sporeforming rodshaped bacterium with peritrichous flagella designated DX5T was isolated from an electroactive biofilm Growth was observed to occur at 35–60 °C at pH 70–100 and with 05–10  w/v NaCl optimum growth 50 °C pH 80 and 05–3  NaCl Cells were determined to be catalase and oxidasepositive The predominant respiratory quinone was identified as MK7 the major polar lipids were determined to be diphosphatidylglycerol phosphatidylglycerol glycolipid aminoglycolipid and an unidentified phospholipid the DNA G+C content was determined to be 466 mol and the major fatty acids 5  were identified as anteisoC150 336  isoC150 241  and anteisoC170 134  The phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain DX5T should be assigned to the genus Bacillus and was related most closely to the type strains of B fortis DSM 16012T 963  B composti KACC 16872T 963  and B fordii DSM 16014T 958  Results of phenotypic chemotaxonomic and genotypic analysis indicated that strain DX5T represents a novel species for which the name B sediminis sp nov is proposed The type strain is DX5T =CGMCC 112412T = KCTC 33102TThis work was supported by the Team Project of Guangdong Natural Science Foundation S2011030002882 and S2012030006114 the Science and Technology Planning Project of Guangdong Province China 2012B030800008 and 2012B010500035 and the Guangzhou Science and Technology Development Foundation China 2011J2200039

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