Emphasis Type="Italic"Novosphingobium aquaticum

Authors: Hina Singh Juan Du JungEun Yang ChangShik Yin MooChang Kook TaeHoo Yi

Publish Date: 2015/07/24

Volume: 108, Issue: 4, Pages: 851-858

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Abstract

A novel Gramstain negative yellow coloured strictly aerobic rodshaped nonmotile bacterium designated as THWSA1T was isolated from lake water near Samsung apartment Suwon Republic of Korea The phylogenetic analysis based on 16S rRNA gene sequences showed that strain THWSA1T belongs to the genus Novosphingobium and is closely related to Novosphingobium taihuense 978  and Novosphingobium subterraneum 971  The DNA–DNA relatedness values between strain THWSA1T and the most closely related type strains were found to be less than 300  The DNA G+C content was determined to be 675 mol The strain grows optimally at 25–28 °C at pH 70 and in the presence of 05  NaCl The predominant isoprenoid quinone was identified as ubiquinone Q10 The polar lipid profile comprises diphosphatidylglycerol phosphatidylglycerol phosphatidylethanolamine phosphatidyldimethylethanolamine sphingoglycolipid phosphatidylcholine some unidentified phospholipids and some unidentified polar lipids Fatty acids characteristic for this genus such as C161 C140 2OH C161 ω6c and/or C161 ω7c summed feature 3 and C181 ω6c and/or C181 ω7c summed feature 8 were also detected On the basis of the phenotypic and genotypic analysis the strain THWSA1T is considered to represent a novel species of the genus Novosphingobium for which the name Novosphingobium aquaticum sp nov is proposed The type strain is THWSA1T =KCTC 42608T=CCTCC AB 2015114T

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