Emphasis Type="Italic"Bacillus/EmphasisEmphas

Authors: Xuexin Wei Di Xin Yuhua Xin Hao Zhang Tianying Wang Jianli Zhang

Publish Date: 2015/10/09

Volume: 109, Issue: 1, Pages: 13-20

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Abstract

A Gramstain positive rodshaped endosporeforming and aerobic bacterium designated BZ1T was isolated from a soil sample collected from a sunflower field in Wuyuan county Inner Mongolia China On the basis of 16S rRNA gene sequence analysis the isolate was found to be a member of the genus Bacillus and the close phylogenetic relatives to be Bacillus gottheilii WCC 4585T Bacillus oceanisediminis H2T Bacillus mesonae FJAT13985T and Bacillus horneckiae DSM 23495T with 983 981 980 and 976  sequence similarity respectively Strain BZ1T was found to grow at 6–40 °C optimum 30–33 °C pH 6090 optimum pH 70 and 0–55  w/v NaCl optimum 05  The cell wall diamino acid of the peptidoglycan of strain BZ1T was identified as mesodiaminopimelic acid and the predominant respiratory quinone as MK7 The major cellular fatty acids were found to be isoC150 anteisoC150 and isoC140 and the polar lipids to consist of diphosphatidylglycerol phosphatidylglycerol and phosphatidylethanolamine The novel strain was found to have a DNA G + C content 445 mol DNA–DNA hybridization with closely related strains was low Based on phenotypic phylogenetic and chemotaxonomic results it is concluded that strain BZ1T represents a novel species within the genus Bacillus for which we propose the name Bacillus depressus sp nov The type strain is BZ1T = CGMCC 115124T = KCTC 33643T

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