Emphasis Type="Italic"Lampropedia puyangensis/E

Authors: Yong Li Tao Wang Chungen Piao Laifa Wang Guozhong Tian Tianhui Zhu Minwei Guo

Publish Date: 2015/05/20

Volume: 108, Issue: 2, Pages: 321-328

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Abstract

A Gramstain negative Neisserstain negative aerobic nonmotile nonsporeforming slimy glossy bacterial strain with single or clustered coccoid cells and white colony colour designated as 2binT was isolated from cankered bark tissue of Populus × euramericana The strain was found to grow at 15–40 °C and pH 5–10 with an optimum of 30 °C and pH 80 The strain was found to be negative with respect to catalase and positive for oxidase activity nitrate reduction and Voges–Proskauer reaction Analysis of 16S rRNA gene sequence data indicated that the isolate belongs to the genus Lampropedia having sequence similarity of 9624  with Lampropedia hyalina ATCC11041T DNA–DNA relatedness of strain 2binT with L hyalina JCM 21380T was 267 ± 46  The DNA G+C content of strain 2binT was determined to be 57  and the major cellular fatty acids were identified as C160 C161 ω7c/C161 ω6c and C181 ω7c The polar lipid profile of strain 2binT was found to contain diphosphatidylglycerol a glycolipid phosphatidylcholine phosphatidylethanolamine phosphatidylglycerol a phospholipid phosphatidylmonomethylethanolamine and three unidentified lipids L1 L2 L3 Based on molecular data and physiological and biochemical characteristics strain 2binT is considered to represent a novel species in the genus Lampropedia for which the name Lampropedia puyangensis sp nov is proposed The type strain is 2binT = CFCC 10925T = KCTC 32235TThe research was supported by the Special Fund of the Chinese Central Government for Basic Scientific Research Operations in Commonweal Research Institutes CAFRIFEEP2011024 and National Infrastructure of Microbial Resources NIMR20137 We also thank Professor Everett M Hansen for his help with the written English language

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