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Title of Journal: Plant Mol Biol Rep

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Abbravation: Plant Molecular Biology Reporter

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Springer-Verlag

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1572-9818

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Highly Conserved UFD1 Proteins Among Eukaryotes Ex

Authors: Lingzhu Wei Ye Tao Haiyan Jia Lixia Zhang Pei Xu Yuezhi Wang Zhengzhi Zhang Caiqin Zhang Zhengqiang Ma
Publish Date: 2009/05/30
Volume: 27, Issue: 4, Pages: 439-447
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Abstract

The UFD1 protein is an important ubiquitin recognition component in the ubiquitinmediated degradation pathway To investigate the conservation of UFD1 genes among eukaryotes and their differentiation two UFD1 paralogs from wheat were identified and mapped to homoeologous chromosome groups 6 and 2 respectively TaUFD1a6B and TaUFD1b2D were cloned and both genes consist of eight introns and of the same intron phases These genes were compared with those in Arabidopsis rice polar yeast and mammals for their sequence chromosome organization and primary protein structure The sequence structure especially those corresponding to the fourth fifth and sixth exons of UFD1 genes is highly conserved across these taxa However unlike yeast and mammals having a single UFD1 gene higher angiosperm species have two ancient UFD1 paralogs Besides the evolutionarily conserved ubiquitinbinding domain at the Nterminus plant UFD1 proteins have three conserved Cterminal motifs Motif I near the UFD1 domain displays a high level of similarity to the mammalian p97binding site and motif III is likely responsible for endoplasmic reticulum membrane retention TaUFD1a6B and TaUFD1b2D are ubiquitously expressed in different plant tissues A green fluorescent proteintransient expression assay in epidermal cells of onion demonstrated that TaUFD1 proteins primarily accumulate in the nucleus

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  1. Genome-Wide Identification of microRNAs and Their Targets in Cold-Stored Potato Tubers by Deep Sequencing and Degradome Analysis
  2. An improved RNA isolation method for succulent plant species rich in polyphenols and polysaccharides
  3. Suppression Subtractive Hybridization Identified Differentially Expressed Genes in Pistil Mutations in Wheat
  4. Genome-wide Comparisons of Gene Expression for Yield Heterosis in Maize
  5. Genome-wide Comparisons of Gene Expression for Yield Heterosis in Maize
  6. Transcriptional Regulation of the Rice Phytase Gene OsPHY1 by Several Phytohormones and Osmotic Stresses Using Promoter-GUS Analysis
  7. Detection of single nucleotide mutations in wheat using single strand conformation polymorphism gels
  8. Wheat Neocentromeres Found in F1 Triticale × Tritordeum Hybrids (AABBRH ch ) After 5-Azacytidine Treatment
  9. BcJMJ30 , the Gene Encoding jmjC Domain-Containing Histone Demethylase Is Associated with Pollen Development and Fertilization in Brassica campestris ssp. chinensis
  10. Differential Protein Phosphorylation Regulates Chloroplast Movement in Response to Strong Light and Darkness in Arabidopsis thaliana
  11. Sulfite Oxidase is Essential for Timely Germination of Maize Seeds upon Sulfite Exposure
  12. Molecular Cloning, Biochemical Characterization, and Differential Expression of an Acetyl-CoA C-Acetyltransferase Gene ( AACT ) of Brahmi ( Bacopa monniera )
  13. Overexpression of the GmNAC2 Gene, an NAC Transcription Factor, Reduces Abiotic Stress Tolerance in Tobacco
  14. A pFBP6::Barnase Construct Resulted in Stigma and Style Ablation and Floral Abscission in Transgenic Tobacco
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  16. Virus-Induced Gene Silencing of a Putative Glutathione S -Transferase Gene Compromised Ol-1 -Mediated Resistance against Powdery Mildew in Tomato
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  20. Isolation and amplification of DNA from rhizomes of turmeric and ginger
  21. A Quantitative Method to Monitor the Efficacy of Inhibitors Against the Chymotrypsin-Like Activity of the Proteasome in Tobacco Leaf Protoplasts
  22. Phylogenetic Analysis of S -Locus Genes Reveals the Complicated Evolution Relationship of S Haplotypes in Brassica
  23. QTL Mapping of Yield and Yield Components under Normal and Salt-stress Conditions in Bread Wheat ( Triticum aestivum L. )
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