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Title of Journal: Plant Mol Biol Rep

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Abbravation: Plant Molecular Biology Reporter

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Springer US

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DOI

10.1016/0273-1177(90)90363-5

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ISSN

1572-9818

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A Novel Coimmunoprecipitation Protocol Based on P

Authors: Jia Wei Yang Jia Xin Fu Jing Li Xiao Ling Cheng Feng Li Jing Fang Dong Zhen Lan Liu Chu Xiong Zhuang
Publish Date: 2013/08/14
Volume: 32, Issue: 1, Pages: 153-161
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Abstract

Protein–protein interactions constitute the regulatory network that coordinates diverse cellular functions Coimmunoprecipitation coIP is a widely used and effective technique to study protein–protein interactions in living cells However the time and cost for the preparation of a highly specific antibody is the major disadvantage associated with this technique In the present study a coIP system was developed to detect protein–protein interactions based on an improved protoplast transient expression system by using commercially available antibodies This coIP system eliminates the need for specific antibody preparation and transgenic plant production Leaf sheaths of rice green seedlings were used for the protoplast transient expression system which demonstrated high transformation and cotransformation efficiencies of plasmids The transient expression system developed by this study is suitable for subcellular localization and protein detection This work provides a rapid reliable and costeffective system to study transient gene expression protein subcellular localization and characterization of protein–protein interactions in vivoThis work was supported by the National Basic Research Program of China 2013CBA01401 Genetically Modified Organisms Breeding Major Projects 2013ZX08001004 the National Natural Science Foundation of China No 30800596 and No 31200191 and China Postdoctoral Science Foundation No 20110490897 We thank Professor LiZhen Tao in South China Agricultural University for help with fluorescent microscope analysis Professor ZhengHui He in San Francisco State University and ShiJiang Zhu in South China Agricultural University for the comments on the manuscript and Cole Johnson in University of Michigan for writing assistance

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  4. Genome-wide Comparisons of Gene Expression for Yield Heterosis in Maize
  5. Genome-wide Comparisons of Gene Expression for Yield Heterosis in Maize
  6. Transcriptional Regulation of the Rice Phytase Gene OsPHY1 by Several Phytohormones and Osmotic Stresses Using Promoter-GUS Analysis
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