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Title of Journal: Plant Mol Biol Rep

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Abbravation: Plant Molecular Biology Reporter

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Springer-Verlag

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DOI

10.1002/mrm.1910380422

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1572-9818

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VirusInduced Gene Silencing of a Putative Glutath

Authors: Dongli Pei Hongzhen Ma Yi Zhang Yuansong Ma Wenjing Wang Huixia Geng Jianyu Wu Chengwei Li
Publish Date: 2011/07/01
Volume: 29, Issue: 4, Pages: 972-978
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Abstract

Previously cDNAamplified fragment length polymorphism analysis was used to profile differentially expressed transcriptderived fragments DETDFs of tomato between resistant and susceptible responses against the fungal pathogen Oidium neolycopersici In the present study we further investigated a DETDF annotated to a putative glutathione Stransferase GST that was more rapidly upregulated during the Ol1mediated resistance response than during the susceptible response We used virusinduced gene silencing to knockdown expression of the putative GST gene ShGST in resistant tomato plants Solanum habrochiates G11560 carrying the Ol1 gene and the ShGSTsilenced plants showed a susceptible phenotype after inoculation with O neolycopersici Microscopic observation demonstrated that O neolycopersici was able to complete its life cycle on silenced resistant plants and showed similar morphological characteristics to those of the fungus on susceptible tomato plants The resistance against O neolycopersici in G11560 was associated with hypersensitive response HR however silencing of ShGST did not completely abolish the HR and hydrogen peroxide H2O2 accumulation These results indicate that ShGST is required for resistance against O neolycopersici in tomato and that decreased HR and H2O2 accumulation may be insufficient for tomato to prevent sporulation of O neolycopersiciWe thank Professor David Baulcombe and Professor Liu Yule for providing the VIGS vectors and Dr Bai Yuling for the seeds of wildtype tomato This research was supported by National Natural Science Foundation of China Projects Grant No 31071807 30600413 Scientific Research Foundation for the Returned Overseas Chinese Scholars State Education Ministry Grant No 20071108 Key Project of Chinese Ministry of Education Grant No 207064 to CL and Natural Science Foundation of Henan Province Grant No 082300430320 to DL

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  2. An improved RNA isolation method for succulent plant species rich in polyphenols and polysaccharides
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  4. Genome-wide Comparisons of Gene Expression for Yield Heterosis in Maize
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