Authors: Yifei Li Lin Qiu Li Huang Jiashu Cao
Publish Date: 2011/09/27
Volume: 30, Issue: 3, Pages: 529-538
Abstract
Histone methylation plays an important role in regulating gene expression and it can act in epigenetic memory system thus deciding fates of cells and their identities The jumonji C jmjC domaincontaining proteins have been shown to reverse histone methylation in nonplant model systems However knowledge of the jmjC domaincontaining histone demethylase that is involved in pollen development and fertilization in plants remains limited In this study BcJMJ30 Brassica campestris Jumonji 30 encoding a putative jmjC domaincontaining protein that is upregulated after pollination of sterile pistils and fertile flower buds has been cloned in the genic male sterile AB line Bajh9701A/B of Chinese cabbagepakchoi B campestris L ssp chinensis Makino Phylogenetic analysis has revealed that the BcJMJ30 was closely related to At3g20810 AtJMJ30 in Arabidopsis which belongs to the JMJD5 subgroup of the jmjC domainonly group Spatial and temporal expression patterns analysis by RTPCR and qRTPCR indicates that transcripts of BcJMJ30 was preferentially expressed in stamens of fertile flower buds and are steadily expressed in pistils following pollination In situ hybridization results have revealed that BcJMJ30 is expressed in both pollen and tapetum In general these results indicated that BcJMJ30 is a novel gene with a jmjC domainonly that may be involved in pollen development and fertilization
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